This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Radzioch, D Articles by Varesio, L Search for Related Content PubMed PubMed Citation Articles by Radzioch, D Articles by Varesio, L

 Previous Article  |  Next Article 

Mol Cell Biol. 1987 February; 7(2): 595-599

Augmentation of c-fos mRNA expression by activators of protein kinase C in fresh, terminally differentiated resting macrophages.

ABSTRACT

Expression of c-fos mRNA was investigated in fresh, normal peritoneal macrophages (M phi), which are terminally differentiated, nonproliferating cells. The levels of c-fos mRNA were dramatically increased by stimulation with phorbol myristate acetate (PMA), calcium ionophore, or 1-oleoyl-2-acetoyl glycerol (OAG). Induction of c-fos mRNA by all the above agents followed similar kinetics, with a peak of mRNA 30 min after stimulation. These results demonstrate that c-fos mRNA can be augmented in fresh, terminally differentiated cells. Since the stimuli increasing c-fos mRNA are direct or indirect activators of protein kinase C, our data suggest that in M phi c-fos mRNA is controlled by protein kinase C activation. PMA, calcium ionophore, and OAG were biologically active in M phi. PMA and calcium ionophore induced respiratory burst and tumoricidal activity, respectively, whereas OAG and PMA were chemotactic for M phi. Interferons beta and gamma, potent M phi activators eliciting tumoricidal activity, did not alter the levels of c-fos mRNA. These results indicate that c-fos mRNA augmentation is a stimulus-specific rather than a function-specific response connected to activation of protein kinase C.

This article has been cited by other articles:

• Reddy, S. P. M., Chuu, Y.-J., Lao, P. N., Donn, J., Ann, D. K., Wu, R. (1995). Expression of Human Squamous Cell Differentiation Marker, SPR1, in Tracheobronchial Epithelium Depends on JUN and TRE Motifs. J. Biol. Chem. 270: 26451-26459 [Abstract] [Full Text]  
• Boulukos, K E, Pognonec, P, Sariban, E, Bailly, M, Lagrou, C, Ghysdael, J (1990). Rapid and transient expression of Ets2 in mature macrophages following stimulation with cMGF, LPS, and PKC activators.. Genes Dev. 4: 401-409 [Abstract]