This Article Full Text (PDF) Other Versions of this Article: MCB.00346-07v1 27/15/5403    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, Y.-C. Articles by Cheng, S.-C. Search for Related Content PubMed PubMed Citation Articles by Liu, Y.-C. Articles by Cheng, S.-C.

 Previous Article  |  Next Article 

Mol. Cell. Biol. doi:10.1128/MCB.00346-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A Novel Splicing Factor Yju2 Is Associated with NTC and Acts after Prp2 in Promoting the First Catalytic Reaction of Pre-mRNA Splicing

Institute of Microbiology and Immunology, National Yang-Ming University, Shih-Pai, Taipei, Taiwan, Republic of China, Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China

	Abstract

The Prp19-associated complex (NTC) is essential for pre-mRNA splicing and is associated with the spliceosome during spliceosome activation. NTC is required for specifying interactions of U5 and U6 with pre-mRNA to stabilize their association with the spliceosome after dissociation of U4. Here we show that a novel splicing factor, Yju2, is associated with components of NTC, and is required for pre-mRNA splicing both in vivo and in vitro. During spliceosome assembly, Yju2 is associated with the spliceosome at nearly the same time as NTC but is destabilized after the first catalytic reaction, whereas other NTC components remain associated until the reaction is complete. Extracts depleted of Yju2 could be complemented by recombinant Yju2, suggesting that Yju2 and NTC are not entirely in association with each other. Yju2 is not required for the binding of NTC to the spliceosome or for NTC-mediated spliceosome activation. Complementation analysis of the affinity-isolated spliceosome formed in Yju2-depleted extracts demonstrated that Yju2 acts in concert with unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome.

This article has been cited by other articles:

• Tsai, R.-T., Tseng, C.-K., Lee, P.-J., Chen, H.-C., Fu, R.-H., Chang, K.-j., Yeh, F.-L., Cheng, S.-C. (2007). Dynamic Interactions of Ntr1-Ntr2 with Prp43 and with U5 Govern the Recruitment of Prp43 To Mediate Spliceosome Disassembly. Mol. Cell. Biol. 27: 8027-8037 [Abstract] [Full Text]