This Article Full Text (PDF) Other Versions of this Article: MCB.00700-06v1 26/21/7942    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, J. Articles by Yamamoto, M. Search for Related Content PubMed PubMed Citation Articles by Zhang, J. Articles by Yamamoto, M.

 Previous Article  |  Next Article 

Mol. Cell. Biol. doi:10.1128/MCB.00700-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

BRG1 Interacts with Nrf2 to Selectively Mediate HO-1 Induction in Response to Oxidative Stress

ERATO Environmental Response Project and Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8577, Japan; Center for Medical Genomics, National Cancer Center Research Institute, 5-1-1 Tsukiji Chuo-ku, Tokyo 104-0045, Japan; Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan

^* To whom correspondence should be addressed. Email: masi{at}tara.tsukuba.ac.jp.

	Abstract

NF-E2 related factor 2 (Nrf2) regulates antioxidant responsive element (ARE)-mediated induction of cytoprotective genes in response to oxidative stress. The purpose of this study is to determine the role of BRG1, a catalytic subunit of SWI2/SNF2-like chromatin remodeling complexes, in Nrf2-mediated gene expression. SiRNA knockdown of BRG1 in SW480 cells selectively decreased inducible expression of heme oxygenase-1 (HO-1) gene after diethylmaleate (DEM) treatment, but did not affect other Nrf2-target genes, such as NAD(P)H:quinone oxidoreductase 1 (NQO1). Chromatin immunoprecipitation analysis revealed that Nrf2 recruits BRG1 to both HO-1 and NQO1 regulatory regions. However, BRG1 knockdown selectively decreased the recruitment of RNA polymerase II to the HO-1, but not to the NQO1 promoter. HO-1, but not other Nrf2-regulated genes, harbors a sequence of TG repeats capable of forming Z-DNA with the BRG1 assistance. Similarly, substitution of the TG repeats with an alternative Z-DNA-forming sequence led to BRG1-mediated activation of HO-1. These results thus demonstrate that BRG1, through the facilitation of Z-DNA formation and subsequent recruitment of RNA polymerase II, is critical in Nrf2-mediated inducible expression of HO-1.

This article has been cited by other articles:

• Takakusa, H., Masumoto, H., Mitsuru, A., Okazaki, O., Sudo, K. (2008). Markers of Electrophilic Stress Caused by Chemically Reactive Metabolites in Human Hepatocytes. Drug Metab. Dispos. 36: 816-823 [Abstract] [Full Text]  
• Kimura, M., Yamamoto, T., Zhang, J., Itoh, K., Kyo, M., Kamiya, T., Aburatani, H., Katsuoka, F., Kurokawa, H., Tanaka, T., Motohashi, H., Yamamoto, M. (2007). Molecular Basis Distinguishing the DNA Binding Profile of Nrf2-Maf Heterodimer from That of Maf Homodimer. J. Biol. Chem. 282: 33681-33690 [Abstract] [Full Text]  
• Cornblatt, B. S., Ye, L., Dinkova-Kostova, A. T., Erb, M., Fahey, J. W., Singh, N. K., Chen, M.-S. A., Stierer, T., Garrett-Mayer, E., Argani, P., Davidson, N. E., Talalay, P., Kensler, T. W., Visvanathan, K. (2007). Preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast. Carcinogenesis 28: 1485-1490 [Abstract] [Full Text]  
• Wong, B., Chen, S., Kwon, J.-A., Rich, A. (2007). Characterization of Z-DNA as a nucleosome-boundary element in yeast Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 104: 2229-2234 [Abstract] [Full Text]