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Mol. Cell. Biol. doi:10.1128/MCB.01063-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A putative Arabidopsis nucleoporin AtNUP160 is critical for RNA export and required for plant tolerance to cold stress

Institute for Integrative Genome Biology, and Department of Botany & Plant Science, University of California at Riverside, Riverside, CA 92521, USA; Department of Plant Sciences, University of Arizona, Tucson, AZ85721, USA

^* To whom correspondence should be addressed. Email: jian-kang.zhu{at}ucr.edu.

	Abstract

To study the genetic control of plant responses to cold stress, Arabidopsis mutants were isolated in a screen for mutations that impair cold-induced transcription of the CBF3-LUC reporter gene. We report here the characterization and cloning of a mutant, atnup160-1, which shows reduced CBF3-LUC induction by cold stress. atnup160-1 mutant plants display altered cold responsive gene expression, and are sensitive to chilling stress and defective in acquired freezing tolerance. AtNUP160 was isolated through positional cloning and shown to encode a putative homolog of animal nucleoporin Nup160. In addition to the impaired expression of CBF genes, microarray analysis revealed that a number of other genes important for plant cold tolerance were also affected in the mutant. The atnup160 mutants flower early, and show retarded seedling growth, especially at low temperatures. AtNUP160 protein is localized at the nuclear rim and Poly(A) mRNA in situ hybridization shows that mRNA export is defective in the atnup160 mutant plants. Our study suggests that the Arabidopsis AtNUP160 is critical for the nucleocytoplasmic transport of mRNAs, and it plays important roles in plant growth and flowering time regulation and is required for cold stress tolerance.

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