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Mol. Cell. Biol. doi:10.1128/MCB.01069-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Different requirements of SWI/SNF complex for the robust nucleosome displacement at promoters of HSF and Msn2/4 regulated heat shock genes

Division of Basic Biomedical Sciences, University of South Dakota, Sanford School of Medicine, Vermillion, South Dakota 57069

^* To whom correspondence should be addressed. Email: aerkine{at}usd.edu.

	Abstract

Stress response in yeast cells is regulated by at least two classes of transcription activators – HSF and Msn2/4, which differentially affect promoter chromatin remodeling. We demonstrate that the deletion of SNF2, an ATPase activity containing subunit of the chromatin remodeling SWI/SNF complex, eliminates histone displacement, RNA polymerase II recruitment, and HSF binding at the HSP12 promoter, while delaying these processes at the HSP82 and SSA4 promoters. Out of the three promoters the double deletion of MSN2 and MSN4 eliminates both chromatin remodeling and HSF binding only at the HSP12 promoter, suggesting that Msn2/4 activators are primary determinants of chromatin disassembly at the HSP12 promoter. Unexpectedly, during heat shock the level of Msn2/4 at the HSP12 promoter declines. This is likely a result of promoter targeted Msn2/4 degradation associated with transcription complex assembly. While histone displacement kinetic profiles bear clear promoter specificity, the kinetic profiles of recovery from heat shock for all analyzed genes display equal or even faster nucleosome return rate, which is to some extent delayed by the deletion of SNF2.