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Mol. Cell. Biol. doi:10.1128/MCB.01504-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II

Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA. The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA. Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104, USA. Center de Regulacio Genomica Dr. Aiguader, 88 08003 Barcelona, Spain

^* To whom correspondence should be addressed. Email: ASH{at}Stowers-Institute.org.

	Abstract

Histone H3Lys27 (H3K27) methylation is a hallmark of silent chromatin, while H3K4 methylation is associated with active chromatin regions. Here, we report that a Drosophila JmjC family member, dUTX, specifically demethylates di- and tri- but not monomethylated H3K27. dUTX localization on chromatin correlates with the elongating form of RNA polymerase II (Pol II) and UTX can associate with Pol II. Furthermore, heat shock induction results in the recruitment of dUTX to the hsp70 gene similar to that of several other Pol II elongation factors. Our data indicate that dUTX is intimately associated with actively transcribed genes and may provide a paradigm for how H3K27 demethylation is required for activation of preinitiated Pol II on transcriptionally poised genes.

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