This Article Full Text (PDF) Other Versions of this Article: MCB.01643-06v1 27/9/3441    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Aronov, S. Articles by Gerst, J. E. Search for Related Content PubMed PubMed Citation Articles by Aronov, S. Articles by Gerst, J. E.

 Previous Article  |  Next Article 

Mol. Cell. Biol. doi:10.1128/MCB.01643-06
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

mRNAs encoding polarity and exocytosis factors are co-transported with cortical ER to the incipient bud in yeast

Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel

^* To whom correspondence should be addressed. Email: jeffrey.gerst{at}weizmann.ac.il.

	Abstract

Polarized growth in yeast depends upon the asymmetric localization and enrichment of polarity/secretion factors at the membrane prior to budding. We examined how these factors (i.e. Cdc42, Sec4, Sro7) reach the bud site and found that their respective mRNAs localize to the tip of the incipient bud prior to nuclear division. Asymmetric mRNA localization depends upon factors that facilitate ASH1 mRNA localization (e.g. 3'-UTR; She proteins 1-5; Puf6; actin cytoskeleton; and a physical association with She2). mRNA placement precedes protein enrichment and subsequent bud emergence, implying that mRNA localization contributes to polarization. Correspondingly, mRNAs encoding proteins which are not asymmetrically distributed (i.e. Snc1, Mso1, Tub1, Pex3, and Oxa1) are not polarized. Finally, mutations which affect cortical ER entry and anchoring in the bud (myo4, sec3, and srp101) also affect asymmetric mRNA localization. Bud-localized mRNAs, including ASH1, were found to co-fractionate with ER microsomes in a She2- and Sec3-dependent manner, thus, asymmetric mRNA transport and cortical ER inheritance are connected processes in yeast.