TABLE 3.
Junctional sequences observed in survivors of the mre11, rad1, rad1 yku70, and dnl4 gene deletion derivatives of SLY19a
| Strain | Junctional sequence | Modifi- cations | No. of overlapping bases | No. of events | |
|---|---|---|---|---|---|
| Parental | TTTATAAAATTATACTGTT | GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGA | |||
| AAATATTTTAATATG | TTGTCATATTAAAATATTTGGGACCAAAACCAAAACATCT | ||||
| JLM30 (mre11Δ) | TTTATAAAATTATA(CTGTT) | (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAG | −5/−5 | 4 | 1 |
| TTTATAAAATTATA(CTGTT) | (AACAGTATAATT)TTATAAACCCTGGTTTTGGTTTTGTAG | −5/−12 | 10 (12) | 2 | |
| TTTATAA(AATTATACTGTT) | (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAG | −12/−5 | 10 (12) | 2 | |
| AAACC(AGGGTTTA--TGTT) | (AACAGTATAATTTTAT)AAACCCTGGTTTTGGTTTTGTAG | −23/−16 | 10 (12) | 4 | |
| TTTATAAAATTATACTG(TT) | (AACAGTATAA---GAGTGGTTGACGAATAATTATG)CTGA | −2/−60 | 9 (10) | 9 | |
| TTTATAAAATTAT(ACTGTT) | (AACAGTATAA---GAGTGGTTGACGAAT)AATTATGCTGA | −6/−53 | 9 (10) | 1 | |
| ACCAAAAACC(AAA--TGTT) | (AACAGTATAATTTTAT)AAACCCTGGTTTTGGTTTTGTAG | −29/−16 | 5 | 1 | |
| EMK13 (rad1Δ) | TTTATAAAATTATACTGTT | (AA)CCAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTA | 0/−2,+1 | 0 | 1 |
| TTTATAAAATTATAC(TGTT) | (A)ACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAG | −4/−1 | 2 | 7 | |
| TTTATAAAATTATACTGT(T) | (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAG | −1/−4 | 2 | 2 | |
| TTTATAAAATTATAC(TGTT) | (AACA)GTATAATTTTATAAACCCTGGTTTTGGTTTTGTAG | −4/−4 | 0 | 5 | |
| TTTATAAAATTA(TACTGTT) | AACAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAGAG | −7/0 | 1 | 1 | |
| TTTATAAAATTATA(CTGTT) | (AACAG)TATAATTTTATAAACCCTGGTTTTGGTTTTGTAG | −5/−5 | 4 | 2 | |
| AACCAG(GGTTT---CTGTT) | (AA)CAGTATAATTTTATAAACCCTGGTTTTGGTTTTGTAG | −21/−2 | 10 (12) | 1 | |
| TTTATAAA(ATTATACTGTT) | (AACAGTATAA)TTTTATAAACCCTGGTTTTGGTTTTGTAG | −11/−11 | 10 (12) | 1 | |
| JLM31 (rad1Δ yku70Δ) | TTTATAAAATTATA(CTGTT) | (AACAGTATAATT)TTATAAACCCTGGTTTTGGTTTTGTAG | −5/−12 | 10 (12) | 1 |
| TTTATAAAATTATACTG(TT) | (AACAGTATAATTTTATAAACC)CTGGTTTTGGTTTTGTAG | −2/−21 | 3 | 1 | |
| AAAACCAGGGTTT(AT--TGTT) | (AACAGTATAATTTTATAAACCCT)GGTTTTGGTTTTGTAG | −16/−23 | 10 (12) | 2 | |
| TTTATAAAATTATACTG(TT) | (AACAGTATAA---GAGTGGTTGACGAATAATTATG)CTGA | −2/−60 | 9 (10) | 3 | |
| JLM32 (dnl4Δ) | TTTATAAAATTATA(CTGTT) | (AACAGTATAATT)TTATAAACCCTGGTTTTGGTTTTGTAG | −5/−12 | 10 (12) | 1 |
| TTTATAAAATTATACTG(TT) | (AACAGTATAA---GAGTGGTTGACGAATAATTATG)CTGA | −2/−60 | 9 (10) | 17 | |
-
↵a Top strand sequences at the sites of joining between two HO breaks in Ura− survivors of the mre11, rad1, rad1 yku70, and dnl4 gene deletion derivatives of SLY19 are shown, as described in Table 2. The insertions are shown in italic.
