Cover photograph (Copyright © 2006, American Society for Microbiology. All Rights Reserved.): The Suppressor of Hairy-wing [Su(Hw)] insulator protein localizes to hundreds of sites in the Drosophila genome that represent putative Su(Hw) insulators (pSIs). Genomic localization of the Su(Hw) protein as visualized by immunostaining with a Su(Hw) antibody (outside image, green) is shown. Immunostaining combined with fluorescence in situ hybridization demonstrates binding of the Su(Hw) protein to pSIs identified using bioinformatics (center). Top, higher magnification of polytene region 62D showing Su(Hw) localization (green); middle, in situ hybridization showing the location of the DNA sequences for pSI 62D (red); bottom, merged image confirming colocalization of the Su(Hw) protein and pSI 62D. (See related article on page 5983.)