Research Articles

• 

  Research Article

  Regional Gene Repression by DNA Double-Strand Breaks in G₁ Phase Cells

  DNA damage responses (DDR) to double-strand breaks (DSBs) alter cellular transcription programs at the genome-wide level. Through processes that are less well understood, DSBs also alter transcriptional responses locally, which may be important for efficient DSB repair. Here, we developed an approach to elucidate the cis-acting responses to DSBs in G₁ phase cells.

  Caitlin E. Purman, Patrick L. Collins, Sofia I. Porter, Ankita Saini, Harshath Gupta, Barry P. Sleckman, Eugene M. Oltz
• 

  Research Article | Spotlight

  Transcriptome-Wide Comparison of Stress Granules and P-Bodies Reveals that Translation Plays a Major Role in RNA Partitioning

  The eukaryotic cytosol contains multiple RNP granules, including P-bodies and stress granules. Three different methods have been used to describe the transcriptome of stress granules or P-bodies, but how these methods compare and how RNA partitioning occurs between P-bodies and stress granules have not been addressed. Here, we compare the analysis of the stress granule transcriptome based on differential centrifugation with and without...

  Tyler Matheny, Bhalchandra S. Rao, Roy Parker
• 

  Research Article

  ZFP281 Recruits MYC to Active Promoters in Regulating Transcriptional Initiation and Elongation

  The roles of the MYC transcription factor in transcriptional regulation have been studied intensively. However, the general mechanism underlying the recruitment of MYC to chromatin is less clear. Here, we found that the Krüppel-like transcription factor ZFP281 plays important roles in recruiting MYC to active promoters in mouse embryonic stem cells.

  Zhuojuan Luo, Xiaoxu Liu, Hao Xie, Yan Wang, Chengqi Lin