Research Articles

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  Research Article

  Meiosis-Specific Functions of Kinesin Motors in Cohesin Removal and Maintenance of Chromosome Integrity in Budding Yeast

  Kinesin motors provide the molecular forces at the kinetochore-microtubule interface and along the spindle to control chromosome segregation. During meiosis with two rounds of microtubule assembly-disassembly, the roles of motor proteins remain unexplored. We observed that in contrast to mitosis, Cin8 and Kip3 together are indispensable for meiosis. While examining meiosis in cin8Δ kip3Δ cells, we detected chromosome...

  Priyanka Mittal, Komal Ghule, Deepika Trakroo, Hemant Kumar Prajapati, Santanu K. Ghosh
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  Research Article

  Ipr1 Regulation by Cyclic GMP-AMP Synthase/Interferon Regulatory Factor 3 and Modulation of Irgm1 Expression via p53

  Intracellular pathogen resistance 1 (Ipr1) has been found to be a mediator to integrate cyclic GMP-AMP synthase (cGAS)–interferon regulatory factor 3 (IRF3), activated by intracellular pathogens, with the p53 pathway. Previous studies have shown the process of Ipr1 induction by various immune reactions, including intracellular bacterial and viral infections. The present study demonstrated that Ipr1 is...

  Fayang Liu, Hongni Xue, Jie Ke, Yongyan Wu, Kezhen Yao, Shuxin Liang, Aihua Xu, Yong Zhang
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  Research Article | Spotlight

  Therapeutic IDOL Reduction Ameliorates Amyloidosis and Improves Cognitive Function in APP/PS1 Mice

  Brain lipoprotein receptors have been shown to regulate the metabolism of ApoE and β-amyloid (Aβ) and are potential therapeutic targets for Alzheimer’s disease (AD). Previously, we identified E3 ubiquitin ligase IDOL as a negative regulator of brain lipoprotein receptors. Genetic ablation of Idol increases low-density lipoprotein receptor protein levels, which facilitates Aβ uptake and clearance by microglia. In this study, we...

  Jie Gao, Russell Littman, Graciel Diamante, Xu Xiao, In Sook Ahn, Xia Yang, Tracy A. Cole, Peter Tontonoz
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  Research Article | Spotlight

  Critical Role of Matrix Metalloproteinase 14 in Adipose Tissue Remodeling during Obesity

  Fibrosis is recognized as the major pathological change in adipose tissue during the development of obesity. However, the detailed mechanisms governing the interactions between the fibrotic components and their modifiers remain largely unclear. Here, we reported that matrix metalloproteinase 14 (MMP14), a key pericellular collagenase, is dramatically upregulated in obese adipose tissue. We generated a doxycycline-inducible adipose...

  Xin Li, Yueshui Zhao, Chuan Chen, Li Yang, Hyun-ho Lee, Zening Wang, Ningyan Zhang, Mikhail G. Kolonin, Zhiqiang An, Xin Ge, Philipp E. Scherer, Kai Sun
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  Research Article

  Cell Cycle-Dependent Switch of TopBP1 Functions by Cdk2 and Akt

  Cdk2-dependent TopBP1-treslin interaction is critical for DNA replication initiation. However, it remains unclear how this association is terminated after replication initiation is finished. Here, we demonstrate that phosphorylation of TopBP1 by Akt coincides with cyclin A activation during S and G₂ phases and switches the TopBP1-interacting partner from treslin to E2F1, which results in the termination of replication...

  Kang Liu, Joshua D. Graves, Yu-Ju Lee, Fang-Tsyr Lin, Weei-Chin Lin