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Research Article

Exocrine pancreas transcription factor 1 binds to a bipartite enhancer element and activates transcription of acinar genes.

S L Weinrich, A Meister, W J Rutter
S L Weinrich
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0534.
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A Meister
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0534.
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W J Rutter
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0534.
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DOI: 10.1128/MCB.11.10.4985
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ABSTRACT

Exocrine pancreas (XP) enhancers, which contain a conserved core sequence, are active only in XP cells. A core enhancer-binding activity also appears to be restricted to XP nuclei. Here we describe the properties of a factor, purified approximately 100,000-fold from pancreas nuclei, which displays core enhancer-binding activity. It is not identical to previously characterized factors and is termed exocrine pancreas transcription factor 1 (XPF-1). In the highly purified preparation, only a single major protein of 60 kDa was detected by silver staining on sodium dodecyl sulfate-gels and by UV cross-linking. XPF-1 binds to the core enhancer of all tested XP genes and not to a mutant sequence which is inactive in vivo. High-affinity binding sites are bipartite. The results of competition binding and UV-cross-linking assays suggest that XPF-1 interacts with both motifs. XPF-1 selectively stimulates transcription of core enhancer templates in an in vitro transcription system. We hypothesize that XPF-1 plays a role in activation of the transcription of XP-specific genes.

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Exocrine pancreas transcription factor 1 binds to a bipartite enhancer element and activates transcription of acinar genes.
S L Weinrich, A Meister, W J Rutter
Molecular and Cellular Biology Oct 1991, 11 (10) 4985-4997; DOI: 10.1128/MCB.11.10.4985

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Exocrine pancreas transcription factor 1 binds to a bipartite enhancer element and activates transcription of acinar genes.
S L Weinrich, A Meister, W J Rutter
Molecular and Cellular Biology Oct 1991, 11 (10) 4985-4997; DOI: 10.1128/MCB.11.10.4985
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