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Research Article

Signals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction.

W Rodgers, B Crise, J K Rose
W Rodgers
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut.
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B Crise
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut.
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J K Rose
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut.
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DOI: 10.1128/MCB.14.8.5384
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ABSTRACT

Glycosyl-phosphatidylinositol (GPI)-anchored membrane proteins and certain protein tyrosine kinases associate with a Triton X-100-insoluble, glycolipid-enriched membrane fraction in MDCK cells. Also, certain protein tyrosine kinases have been shown to associate with GPI-anchored proteins in other cell types. To characterize the interaction between GPI-anchored proteins and protein tyrosine kinases, GPI-anchored proteins were coexpressed with p56lck in HeLa cells. Both proteins were shown to target independently to the glycolipid-enriched membranes. Coimmunoprecipitation of GPI-anchored proteins and p56lck occurred only when both proteins were located in the glycolipid-enriched membranes, and gentle disruption of these membranes abolished the interaction. The GPI anchor was found to be the targeting signal for this membrane fraction in GPI-anchored proteins. Analysis of mutants indicated that p56lck was nearly quantitatively palmitoylated at Cys-5 but not palmitoylated at Cys-3. The nonpalmitoylated cysteine at position 3 was very important for association of p56lck with the membrane fraction, while palmitoylation at Cys-5 promoted only a low level of interaction. Because other src family protein tyrosine kinases that are associated with GPI-anchored proteins always contain a Cys-3, we propose that this residue, in addition to the N-terminal myristate, is part of a common signal targeting these proteins to a membrane domain that has been linked to transmembrane signaling.

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Signals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction.
W Rodgers, B Crise, J K Rose
Molecular and Cellular Biology Aug 1994, 14 (8) 5384-5391; DOI: 10.1128/MCB.14.8.5384

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Signals determining protein tyrosine kinase and glycosyl-phosphatidylinositol-anchored protein targeting to a glycolipid-enriched membrane fraction.
W Rodgers, B Crise, J K Rose
Molecular and Cellular Biology Aug 1994, 14 (8) 5384-5391; DOI: 10.1128/MCB.14.8.5384
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