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Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.

Repression of major histocompatibility complex I-A beta gene expression by dbpA and dbpB (mYB-1) proteins.

J Lloberas, R A Maki, A Celada
J Lloberas
Departament de Fisiologia (Immunologia), Facultat de Biologia, Barcelona, Spain.
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R A Maki
Departament de Fisiologia (Immunologia), Facultat de Biologia, Barcelona, Spain.
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A Celada
Departament de Fisiologia (Immunologia), Facultat de Biologia, Barcelona, Spain.
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DOI: 10.1128/MCB.15.9.5092
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ABSTRACT

The induction of major histocompatibility complex class II gene expression is mediated by three DNA elements in the promoters of these genes (W, X, and Y boxes). The Y box contains an inverted CCAAT box sequence, and the binding activity to the CAAT box is mediated by factor NF-Y, which is composed of subunits NF-YA and NF-YB. We have found that transfection of either dbpA or dbpB (mYB-1) or both inhibits I-A beta gene expression. Although the genes for some members of the Y-box family of binding proteins have been isolated by screening an expression library using the Y-box sequence, under our conditions no binding of dbpA or dbpB to the Y box of the I-A beta or I-E alpha promoter was detected. This suggested that repression of I-A beta gene expression by dbpA and dbpB was not due to competition for binding to the Y-box sequence. The results suggest two other mechanisms by which dbpA and dbpB can inhibit transcription from the I-A beta promoter. When dbpA was added, the binding of NF-YA to DNA increased, which could be explained by interaction between these two proteins whose purpose is to increase the binding affinity of NF-YA for DNA. However, this complex was unable to stimulate transcription from the I-A beta promoter. Thus, dbpA competed for the interaction between NF-YA and NF-YB by binding to NF-YA. When dbpB factor was added together with NF-YA and NF-YB, the binding of the NF-YA--NF-YB complex was reduced. This suggested that dbpB may complete with NF-YB for interaction with NF-YA. These results provide an example of how dbpA and dbpB may regulate transcription of promoters that utilize NF-Y as a transcription factor.

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Repression of major histocompatibility complex I-A beta gene expression by dbpA and dbpB (mYB-1) proteins.
J Lloberas, R A Maki, A Celada
Molecular and Cellular Biology Sep 1995, 15 (9) 5092-5099; DOI: 10.1128/MCB.15.9.5092

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Repression of major histocompatibility complex I-A beta gene expression by dbpA and dbpB (mYB-1) proteins.
J Lloberas, R A Maki, A Celada
Molecular and Cellular Biology Sep 1995, 15 (9) 5092-5099; DOI: 10.1128/MCB.15.9.5092
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