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GENE EXPRESSION

Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing

Sarah J. Lee, Susan J. Baserga
Sarah J. Lee
Department of Therapeutic Radiology and Genetics and
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Susan J. Baserga
Department of Therapeutic Radiology and Genetics and
Yale Cancer Center, Yale School of Medicine, New Haven, Connecticut 06520-8040
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DOI: 10.1128/MCB.19.8.5441
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ABSTRACT

The function of the U3 small nucleolar ribonucleoprotein (snoRNP) is central to the events surrounding pre-rRNA processing, as evidenced by the severe defects in cleavage of pre-18S rRNA precursors observed upon depletion of the U3 RNA and its unique protein components. Although the precise function of each component remains unclear, since U3 snoRNA levels remain unchanged upon genetic depletion of these proteins, it is likely that the proteins themselves have significant roles in the cleavage reactions. Here we report the identification of two previously undescribed protein components of the U3 snoRNP, representing the first snoRNP components identified by using the two-hybrid methodology. By screening for proteins that physically associate with the U3 snoRNP-specific protein, Mpp10p, we have identified Imp3p (22 kDa) and Imp4p (34 kDa) (named for interacting with Mpp10p). The genes encoding both proteins are essential in yeast. Genetic depletion reveals that both proteins are critical for U3 snoRNP function in pre-18S rRNA processing at the A0, A1, and A2 sites in the pre-rRNA. Both Imp proteins associate with Mpp10p in vivo, and both are complexed only with the U3 snoRNA. Conservation of RNA binding domains between Imp3p and the S4 family of ribosomal proteins suggests that it might associate with RNA directly. However, as with other U3 snoRNP-specific proteins, neither Imp3p nor Imp4p is required for maintenance of U3 snoRNA integrity. Imp3p and Imp4p are therefore novel protein components specific to the U3 snoRNP with critical roles in pre-rRNA cleavage events.

  • Copyright © 1999 American Society for Microbiology
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Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing
Sarah J. Lee, Susan J. Baserga
Molecular and Cellular Biology Aug 1999, 19 (8) 5441-5452; DOI: 10.1128/MCB.19.8.5441

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Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing
Sarah J. Lee, Susan J. Baserga
Molecular and Cellular Biology Aug 1999, 19 (8) 5441-5452; DOI: 10.1128/MCB.19.8.5441
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KEYWORDS

RNA Precursors
RNA, Fungal
RNA, Ribosomal, 18S
Ribonucleoproteins, Small Nuclear
ribosomal proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins

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