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TRANSCRIPTIONAL REGULATION

Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF

E. Kelly Sullivan, Christine S. Weirich, Jeffrey R. Guyon, Saı̈d Sif, Robert E. Kingston
E. Kelly Sullivan
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, 1 and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 2
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Christine S. Weirich
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, 1 and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 2
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Jeffrey R. Guyon
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, 1 and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 2
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Saı̈d Sif
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, 1 and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 2
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Robert E. Kingston
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, 1 and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 2
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DOI: 10.1128/MCB.21.17.5826-5837.2001
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ABSTRACT

Chromatin remodeling complexes such as SWI/SNF use the energy of ATP hydrolysis to remodel nucleosomal DNA and increase transcription of nucleosomal templates. Human heat shock factor one (hHSF1) is a tightly regulated activator that stimulates transcriptional initiation and elongation using different portions of its activation domains. Here we demonstrate that hHSF1 associates with BRG1, the ATPase subunit of human SWI/SNF (hSWI/SNF) at endogenous protein concentrations. We also show that hHSF1 activation domains recruit hSWI/SNF to a chromatin template in a purified system. Mutation of hHSF1 residues responsible for activation of transcriptional elongation has the most severe effect on recruitment of SWI/SNF and association of hHSF1 with BRG1, suggesting that recruitment of chromatin remodeling activity might play a role in stimulation of elongation.

  • Copyright © 2001 American Society for Microbiology
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Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF
E. Kelly Sullivan, Christine S. Weirich, Jeffrey R. Guyon, Saı̈d Sif, Robert E. Kingston
Molecular and Cellular Biology Sep 2001, 21 (17) 5826-5837; DOI: 10.1128/MCB.21.17.5826-5837.2001

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Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF
E. Kelly Sullivan, Christine S. Weirich, Jeffrey R. Guyon, Saı̈d Sif, Robert E. Kingston
Molecular and Cellular Biology Sep 2001, 21 (17) 5826-5837; DOI: 10.1128/MCB.21.17.5826-5837.2001
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