pHT1 | N2301 | 26 | AATAAATAAATAAATTTTTTAAAATA | Random, 100% A:T | 37.0 | 1.8 |

| | | TTATTTATTTATTTAAAAAATTTTAT | | | |

pHT2 | N2302 | 25 | TTTTTTAATTTTATTTTAAATTTTT | Random, 100% A:T | 6.6 | 1.2 |

| | | AAAAAATTAAAATAAAATTTAAAAA | | | |

pHT3 | N2303 | 24 | AATTTTTTATTTATTATATAATTT | Random, 100% A:T | 3.2 | 1.3 |

| | | TTAAAAAATAAATAATATATTAAA | | | |

pHT4 | N2304 | 23 | TAAAAATTAAAATAATTAAAAAA | Random, 100% A:T | 6.8 | 1.1 |

| | | ATTTTTAATTTTATTAATTTTTT | | | |

pHT5 | N2305 | 25 | ATTTTAAAAAAAATATATTAATAAA | Random, 100% A:T | 14.5 | 0.1 |

| | | TAAAATTTTTTTTATATAATTATTT | | | |

pHT6 | N2306 | 24 | ATTATATATTTTATTAATTTTTTT | Random, 100% A:T | 6.3 | 0.9 |

| | | TAATATATAAAATAATTAAAAAAA | | | |

pHT7 | N2307 | 25 | TTATTATTTAAATTTTAAATAAATT | Random, 100% A:T | 15.2 | 2.8 |

| | | AATAATAAATTTAAAATTTATTTAA | | | |

pHT8 | N2308 | 25 | TAAATTTAATAAATTTATTTTTAAT | Random, 100% A:T | 27.0 | 8.1 |

| | | ATTTAAATTATTTAAATAAAAATTA | | | |

pHT9 | N2309 | 23 | TAAAAATTAAAATAATTAAAAAA | Random, 100% A:T | 9.0 | 2.9 |

| | | ATTTTTAATTTTATTAATTTTTT | | | |

pHT10 | N2310 | 25 | TTTATTTAAATTTATAATAATTATA | Random, 100% A:T | 14.7 | 0.7 |

| | | AAATAAATTTAAATATTATTAATAT | | | |

pHT11 | N2311 | 25 | TATTATAATATTTATAATTATTTTT | Random, 100% A:T | 8.7 | 1.1 |

| | | ATAATATTATAAATATTAATAAAAA | | | |

pHT12 | N2312 | 25 | TATTATAATATTTATAATTATTTTT | Random, 100% A:T | 5.7 | 0.2 |

| | | ATAATATTATAAATATTAATAAAAA | | | |

pHT13 | N2313 | 25 | AAATAATAAAAAAATATTTTTTTAA | Random, 100% A:T | 9.0 | 1.5 |

| | | TTTATTATTTTTTTATAAAAAAATT | | | |

pHT14 | N2314 | 25 | TAAAATATTATTTATATTAATAAAA | Random, 100% A:T | 7.1 | 0.7 |

| | | ATTTTATAATAAATATAATTATTTT | | | |

pHT15 | N2315 | 25 | AATAAAAAATAAATAATTATAATAA | Random, 100% A:T | 12.1 | 3.8 |

| | | TTATTTTTTATTTATTAATATTATT | | | |

pHT16 | N2316 | 25 | AAAATTTTAAATATATAATAATAAT | Random, 100% A:T | 16.2 | 5.7 |

| | | TTTTAAAATTTATATATTATTATTA | | | |

pHT17 | N2317 | 25 | AATAAAAATATTTTTATTTATAAAA | Random, 100% A:T | 7.4 | 2.8 |

| | | TTATTTTTATAAAAATAAATATTTT | | | |

pHT18 | N2318 | 25 | TAATATATATAAAAAATATAAATTA | Random, 100% A:T | 6.1 | 2.3 |

| | | ATTATATATATTTTTTATATTTAAT | | | |

pHT19 | N2319 | 25 | TATAAATTTATTTATATAATTAAAA | Random, 100% A:T | 12.2 | 0.6 |

| | | ATATTTAAATAAATATATTAATTTT | | | |

pHT20 | N2320 | 25 | GTAGGACTTACACCACCTGCGGGTC | Random, 50% G:C | 0.0 | |

| | | CATCCTGAATGTGGTGGACGCCCAG | | | |

pHT21 | N2321 | 23 | GGACGACCCGATACCACGCGACA | Random, 50% G:C | 0.0 | |

| | | CCTGCTGGGCTATGGTGCGCTGT | | | |

pHT22 | N2322 | 25 | CGCAGTTGCCCCAAATAGGAGGGAT | Random, 50% G:C | 0.0 | |

| | | GCGTCAACGGGGTTTATCCTCCCTA | | | |

pHT23 | N2323 | 25 | CACCAGCATCATGGTGCGCCTCAAG | Random, 50% G:C | 0.0 | |

| | | GTGGTCGTAGTACCACGCGGAGTTC | | | |

pHT24 | N2324 | 24 | CGGTGACGGTGAACAAGCTCAACA | Random, 50% G:C | 0.0 | |

| | | GCCACTGCCACTTGTTCGAGTTGT | | | |

pHT25 | N2325 | 25 | GACTCGTGCCAAGTCTTCAAGGTCC | Random, 50% G:C | 0.0 | |

| | | CTGAGCACGGTTCAGAAGTTCCAGG | | | |

pHT26 | N2326 | 21 | TAGCGGAGATCACCCCCAATC | Random, 50% G:C | 0.0 | |

| | | ATCGCCTCTAGTGGGGGTTAG | | | |

pHT27 | N2327 | 25 | GGTAGCCACGGGCCTTGCCAAGCTT | Random, 50% G:C | 0.0 | |

| | | CCATCGGTGCCCGGAACGGTTCGAA | | | |

pHT28 | N2328 | 25 | TATCCAGACGGACACACGGCAACTT | Random, 50% G:C | 0.0 | |

| | | ATAGGTCTGCCTGTGTGCCGTTGAA | | | |

pHT29 | N2329 | 24 | TTTCGCTCTGCGCGGCTCATAAAT | Random, 50% G:C | 0.0 | |

| | | AAAGCGAGACGCGCCGAGTATTTA | | | |

pHT30 | N2330 | 25 | TGGGCCGGCGAAGTTGGTAGAGGGT | Random, 50% G:C | 0.0 | |

| | | ACCCGGCCGCTTCAACCATCTCCCA | | | |

pHT31 | N2331 | 25 | CTGCACACGTCATAATCCTGGGAGG | Random, 50% G:C | 0.0 | |

| | | GACGTGTGCAGTATTAGGACCCTCC | | | |

pHT32 | N2332 | 25 | AAAAAAAAAAAAAAAAAAAAAAAAA | A | 2.1 | 0.6 |

| N2333 | | TTTTTTTTTTTTTTTTTTTTTTTTT | T | | |

pHT33 | N2334 | 25 | AATAAAAAATAAAAAATAAAAAATA | TAAAAAA | 10.2 | 0.7 |

| N2335 | | TTATTTTTTATTTTTTATTTTTTAT | ATTTTTT | | |

pHT34 | N2336 | 25 | AATAAAAATAAAAATAAAAATAAAA | TAAAAA | 10.4 | 1.3 |

| N2337 | | TTATTTTTATTTTTATTTTTATTTT | ATTTTT | | |

pHT35 | N2338 | 25 | AATAAAATAAAATAAAATAAAATAA | TAAAA | 12.8 | 1.6 |

| N2339 | | TTATTTTATTTTATTTTATTTTATT | ATTTT | | |

pHT36 | N2340 | 25 | AATAAATAAATAAATAAATAAATAA | TAAA | 29.7 | 4.3 |

| N2341 | | TTATTTATTTATTTATTTATTTATT | ATTT | /PICK> | |

Plasmid designation | *N. crassa* strain(s) with targeted sequence(s) | Length tested (bp) | Insert sequence | Repeat unit or feature | Methylation^{b} (%) |

Mean | SD |

pHT37 | N2342 | 25 | AATTAATTAATTAATTAATTAATTA | AATT | 24.9 | 1.8 |

| N2343 | | TTAATTAATTAATTAATTAATTAAT | TTAA | | |

pHT38 | N2344 | 25 | AATAATAATAATAATAATAATAATA | TAA | 10.8 | 1.0 |

| N2345 | | TTATTATTATTATTATTATTATTAT | ATT | | |

pHT39 | N2346 | 25 | AATATATATATATATATATATATAT | TA | 5.8 | 2.2 |

| N2347 | | TTATATATATATATATATATATATATATA | AT | | |

pHT40 | N2348 | 20 | TAAATAAATAAATAAATAAA | TAAA | 11.4 | 5.2 |

| N2349 | | ATTTATTTATTTATTTATTT | ATTT | | |

pHT41 | N2350 | 40 | TAAATAAATAAATAAATAAA... | TAAA | 74.4 | 5.9 |

| N2351 | | ATTTATTTATTTATTTATTT... | ATTT | | |

pHT42 | N2352 | 80 | TAAATAAATAAATAAATAAATAAAT... | TAAA | 81.4 | 1.3 |

| N2353 | | ATTTATTTATTTATTTATTTATTTA... | ATTT | | |

pHT43 | N2354 | 25 | TAAAATAAAATAAAATAAAATAAAA | TAAAA | 11.6 | 0.2 |

| N2355 | | ATTTTATTTTTATTTTATTTTTATTTT | ATTTT | | |

pHT44 | N2356 | 25 | CTAAACTAAACTAAACTAAACTAAA | CTAAA | 1.5 | 0.2 |

| N2357 | | GATTTGATTTGATTTGATTTGATTT | GATTT | | |

pHT45 | N2358 | 25 | CAAATCAAATCAAATCAAATCAAAT | TCAAA | 1.2 | 0.1 |

| N2359 | | GTTTAGTTTAGTTTAGTTTAGTTTA | AGTTT | | |

pHT46 | N2360 | 25 | CAATACAATACAATACAATACAATA | TACAA | 0.5 | 0.1 |

| N2361 | | GTTATGTTATGTTATGTTATGTTAT | ATGTT | | |

pHT47 | N2362 | 25 | CATAACATAACATAACATAACATAA | TAACA | 0.3 | 0.2 |

| N2363 | | GTATTGTATTGTATTGTATTGTATT | ATTGT | | |

pHT48 | N2364 | 100 | CAAAACAAAACAAAACAAAACAAAA... | CAAAA | 4.2 | 1.3 |

| N2365 | | GTTTTGTTTTGTTTTGTTTTGTTTT... | GTTTT | | |

pHT49 | N2366 | 100 | CTAAACTAAACTAAACTAAACTAAA... | CTAAA | 63.7 | 6.6 |

| N2367 | | GATTTGATTTGATTTGATTTGATTT... | GATTT | | |

pHT50 | N2368 | 100 | CAAATCAAATCAAATCAAATCAAAT... | TCAAA | 86.2 | 6.2 |

| N2369 | | GTTAGTTTAGTTTAGTTTAGTTTA... | AGTTT | | |

pHT51 | N2370 | 100 | GAAATGAAATGAAATGAAATGAAAT... | TGAAA | 8.0 | 1.2 |

| N2371 | | CTTTACTTTACTTTACTTTACTTTA... | ACTTT | | |

pHT52 | N2372 | 100 | CAATACAATACAATACAATACAATA... | TACAA | 34.8 | 8.3 |

| N2373 | | GTTATGTTATGTTATGTTATGTTAT... | ATGTT | | |

pHT53 | N2374 | 100 | CATAACATAACATAACATAACATAA... | TAACA | 5.2 | 0.3 |

| N2375 | | GTATTGTATTGTATTGTATTGTATT... | ATTGT | | |

pHT54 | N2376 | 100 | CTAATCTAATCTAATCTAATCTAAT... | CTAAT | 24.4 | 10.5 |

| N2377 | | GATTAGATTAGATTAGATTAGATTA... | GATTA | | |

pHT55 | N2378 | 100 | CTAAAACTAAAACTAAAACTAAAAA... | CTAAAA | 74.7 | 1.3 |

| N2379 | | GATTTTGATTTTGATTTTGATTTTT... | GATTTT | | |

pHT56 | N2380 | 100 | TCAAAATCAAAATCAAAATCAAAAT... | TCAAAA | 70.6 | 4.9 |

| N2381 | | AGTTTTAGTTTTAGTTTTAGTTTTA... | AGTTTT | | |

pHT57 | N2382 | 100 | CAAACAAACAAACAAACAAACAAAC... | CAAA | 1.33 | 0.7 |

| N2383 | | GTTTGTTTGTTTGTTTGTTTGTTTG... | GTTT | | |

pHT58 | N2384 | 100 | CTAACTAACTAACTAACTAACTAAC... | CTAA | 22.2 | 3.4 |

| N2385 | | GATTGATTGATTGATTGATTGATTG... | GATT | | |

pHT59 | N2386 | 100 | CAATCAATCAATCAATCAATCAATC... | TCAA | 45.3 | 6.7 |

| N2387 | | GTTAGTTAGTTAGTTAGTTAGTTAG... | AGTT | | |

pHT60 | N2388 | 100 | CTTACTTACTTACTTACTTACTTAC... | GTAA | 10.9 | 1.5 |

| N2389 | | GAATGAATGAATGAATGAATGAATG... | CATT | | |

pHT61 | N2390 | 100 | CATTCATTCATTCATTCATTCATTC... | TGAA | 0.7 | 0.4 |

| N2391 | | GTAAGTAAGTAAGTAAGTAAGTAAG... | ACTT | | |

pHT62 | N2392 | 100 | CTATCTATCTATCTATCTATCTATC... | CTAT | 9.1 | 1.6 |

| N2393 | | GATAGATAGATAGATAGATAGATAG... | GATA | | |

pHT63 | N2394 | 100 | CAACAACAACAACAACAACAACAAC... | CAA | 0.0 | |

| N2395 | | GTTGTTGTTGTTGTTGTTGTTGTTG... | GTT | | |

pHT64 | N2396 | 100 | CTTCTTCTTCTTCTTCTTCTTCTTC... | GAA | 0.2 | 0.1 |

| N2397 | | GAAGAAGAAGAAGAAGAAGAAGAAG... | CTT | | |

pHT65 | N2398 | 100 | CTACTACTACTACTACTACTACTAC... | CTA | 32.3 | 6.0 |

| N2399 | | GATGATGATGATGATGATGATGATG... | GAT | | |

pHT66 | N2400 | 100 | CATCATCATCATCATCATCATCATC... | CAT | 0.0 | |

| N2401 | | GTAGTAGTAGTAGTAGTAGTAGTAG... | GTA | | |

pHT67 | N2402 | 100 | GATCGATCGATCGATCGATCGATCG... | GATC | 5.7 | 0.8 |

| N2403 | | CTAGCTAGCTAGCTAGCTAGCTAGC... | CTAG | | |

pHT68 | N2404 | 100 | CATGCATGCATGCATGCATGCATGC... | CATG | 0.0 | |

| N2405 | | GTACGTACGTACGTACGTACGTACG... | GTAC | | |

pHT69 | N2406 | 100 | CTAGCTAGCTAGCTAGCTAGCTAGC... | CTAG | 1.7 | 0.4 |

| N2407 | | GATCGATCGATCGATCGATCGATCG... | GATC | | |