Article Figures & Data
Figures
- FIG. 1.
Mouse knockout construct and verification of TIEG1 null mutation. (A) Generation of the TIEG1 null allele (bottom panel) by homologous recombination between the targeting vector (middle panel) and wild-type allele (top panel). In the recombinant allele, the 1.8-kb Neor cassette replaces ca. 5.5 kb of genomic TIEG1 sequence, including exons 1 and 2. The locations of the 5′ and 3′ probes used in Southern analysis screening of transfected ES cell clones are indicated in the top panel. (B) PCR genotyping of mice generated from the mating of heterozygous TIEG1 mutant mice. The arrows indicate the products produced from the wild-type and mutant alleles. (C) Southern blot analysis was performed on genomic DNA from wild-type (TIEG+/+), heterozygous (TIEG+/−), and homozygous (TIEG−/−) TIEG1 mutant mice to verify genotypes. (D) In order to verify loss of TIEG1 expression, Northern analysis was performed as described in Materials and Methods. RNA was extracted from OBs grown in serum-free medium and treated with 2 ng of TGF-β/ml indicated times. (E) Immunoprecipitation analysis was performed from 35S-labeled cell lysates with TIEG-specific polyclonal antibody to demonstrate that TIEG−/− mice did not express TIEG1 protein.
- FIG. 2.
Histomorphometric analysis of mice. (A) Cell count for osteoblast and osteoclasts per surface area in cancellous bone (in millimeters). (B) Tissue and cellular measurements. The units differ for each data set and were as follows: LS/BS, the percentage of labeled cancellous bone surface per total measured cancellous bone surface; BFR/BS, the amount of new bone formed per week expressed as the percentage of bone surface; BFR/TV, the amount of new bone formed per week expressed as a percentage of total tissue volume; BV/TV, the percentage of cancellous bone volume per measured tissue volume; MAR, mineral apposition rate (>1 μm/week). Data are the means of 5 (+/+) or 6 (−/−) animals ± the SEM. ❋, P < 0.05.
- FIG. 3.
TIEG+/+ and TIEG−/− OBs were plated onto 96-well plates and the cells were grown for 24, 48, and 72 h at 37°C. The proliferation of these cells were measured by using cell titer 96 “the Aqueous One solution for cell proliferation assay” as described in Materials and Methods. An average of six replicates of each were graphed.
- FIG. 4.
TIEG+/+ and TIEG−/− OBs were grown in differentiation medium with or without BMP2 for 18 days and stained with alizarin red to visualize the bone nodules.
- FIG. 5.
Total RNA was isolated from TIEG+/+ and TIEG−/− OBs grown in culture, and RT-PCR was performed for osteoblast-specific marker genes. (A) Agarose gel of the RT-PCR products as visualized with ethidium bromide. (B) RT-PCR was performed on five separate calvarial RNA isolates, and the results were scanned and quantitated by using NIH Image. The data are the means ± the SEM of these analyses normalized to GAPDH expression. ❋, P < 0.05 (comparing TIEG+/+ to TIEG−/− OBs).
- FIG. 6.
Knockout OBs are impaired in supporting osteoclast differentiation. Neonatal calvarium-derived osteoblasts from TIEG+/+ and TIEG−/− mice were cultured with either marrow or spleen osteoclast precursors from TIEG+/+ mice in the presence of vitamin D and dexamethasone for 9 days as described in the text. The data are means ± the SEM of three replicate wells from one experiment. The experiment was performed four times, and these data are representative of the results. ❋, P < 0.05 (comparing TIEG+/+ to TIEG−/− calvarial cells).
- FIG. 7.
The RANKL/OPG ratio is lower in TIEG−/− cells than in TIEG+/+ cells. Calvarial cells were cultured with (treated) vitamin D and dexamethasone or without treatment (control), followed by RNA isolation as described in the text. Real-time PCR was performed to quantitate the M-CSF, RANKL, and OPG expression relative to tubulin, and the ratio of RANKL to OPG was determined. ❋, P < 0.05 (comparing TIEG+/+ to TIEG−/− calvarial cells).
- FIG. 8.
RANKL partly, but not completely, restores TIEG−/− calvarial cell ability to support osteoclast differentiation. TIEG+/+ and TIEG−/− calvarial cells were cultured with TIEG+/+ marrow in the presence of the hormones and/or growth factors indicated at the bottom of the figure. ❋, P < 0.05 (comparing TIEG+/+ to TIEG−/− calvarial OB cells); §, P < 0.05 [comparing vitamin D and dexamethasone alone to addition of the indicated growth factor(s)].
