DOI: 10.1128/MCB.01470-07
Volume 26, no. 23, p. 9083-9093, 2006. As stated in Materials and Methods, the volume of reagents microinjected into embryos in knockdown and rescue experiments varied between 1 and 3 nl. However, it is important to note that the amounts of microinjected morpholino oligonucleotides or RNA provided throughout the paper were calculated with the assumption that a volume of 3 nl was injected in each case.
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