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Research Article

Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts.

C S Lin, R H Aebersold, S B Kent, M Varma, J Leavitt
C S Lin
Institute for Medical Research, San Jose, California 95128.
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R H Aebersold
Institute for Medical Research, San Jose, California 95128.
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S B Kent
Institute for Medical Research, San Jose, California 95128.
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M Varma
Institute for Medical Research, San Jose, California 95128.
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J Leavitt
Institute for Medical Research, San Jose, California 95128.
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DOI: 10.1128/MCB.8.11.4659
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ABSTRACT

The phosphoprotein plastin was originally identified as an abundant transformation-induced polypeptide of chemically transformed neoplastic human fibroblasts. This abundant protein is normally expressed only in leukocytes, suggesting that it may play a role in hemopoietic cell differentiation. Protein microsequencing of plastin purified from leukemic T lymphocytes by high-resolution two-dimensional gel electrophoresis produced eight internal oligopeptide sequences. An oligodeoxynucleotide probe corresponding to one of the oligopeptides was used to clone cDNAs from transformed human fibroblasts that encoded the seven other oligopeptides predicted for human plastin. Sequencing and characterization of two cloned cDNAs revealed the existence of two distinct, but closely related, isoforms of plastin--l-plastin, which is expressed in leukocytes and transformed fibroblasts, and t-plastin, which is expressed in normal cells of solid tissues and transformed fibroblasts. The leukocyte isoform l-plastin is expressed in a diverse variety of human tumor cell lines, suggesting that it may be involved in the neoplastic process of some solid human tumors.

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Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts.
C S Lin, R H Aebersold, S B Kent, M Varma, J Leavitt
Molecular and Cellular Biology Nov 1988, 8 (11) 4659-4668; DOI: 10.1128/MCB.8.11.4659

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Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts.
C S Lin, R H Aebersold, S B Kent, M Varma, J Leavitt
Molecular and Cellular Biology Nov 1988, 8 (11) 4659-4668; DOI: 10.1128/MCB.8.11.4659
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