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Research Article

S-phase-specific transcription regulatory elements are present in a replication-independent testis-specific H2B histone gene.

I Hwang, C B Chae
I Hwang
Department of Biochemistry, University of North Carolina, Chapel Hill 27599-7260.
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C B Chae
Department of Biochemistry, University of North Carolina, Chapel Hill 27599-7260.
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DOI: 10.1128/MCB.9.3.1005
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ABSTRACT

The testis-specific H2B histone (TH2B) gene is expressed in pachytene spermatocytes during meiotic prophase I in the absence of any significant DNA synthesis. Unlike somatic histones, synthesis of testis-specific histones is not affected by inhibitors of DNA synthesis. A genomic rat TH2B gene was cloned by using a DNA fragment derived from TH2B cDNA as a probe. Expression of the cloned TH2B was investigated by gene transfer experiments. From these studies, we found that the 5' upstream region of the cloned TH2B gene contained S-phase-specific transcription elements which regulated expression of a reporter gene in an S-phase-specific manner. The S-phase-regulatory element was found to be located in two regions containing CCAAT elements between -153 and -110 base pairs (bp) and an octamer element (ATTTGCAT) between -109 and -84 bp. The two regions were required for a maximal stimulation of transcription of the cloned TH2B gene in S phase. On the other hand, only the octamer element was reported be important for the S-phase-specific transcription of human H2B gene. Since the synthesis of the TH2B histone is independent of DNA synthesis and specific for pachytene spermatocytes in vivo, the presence of the S-phase-specific transcription regulatory elements in the TH2B gene is surprising.

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S-phase-specific transcription regulatory elements are present in a replication-independent testis-specific H2B histone gene.
I Hwang, C B Chae
Molecular and Cellular Biology Mar 1989, 9 (3) 1005-1013; DOI: 10.1128/MCB.9.3.1005

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S-phase-specific transcription regulatory elements are present in a replication-independent testis-specific H2B histone gene.
I Hwang, C B Chae
Molecular and Cellular Biology Mar 1989, 9 (3) 1005-1013; DOI: 10.1128/MCB.9.3.1005
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