PT  - JOURNAL ARTICLE
AU  - Eapen, Vinay V.
AU  - Sugawara, Neal
AU  - Tsabar, Michael
AU  - Wu, Wei-Hua
AU  - Haber, James E.
TI  - The <span class="named-content genus-species" id="named-content-1">Saccharomyces cerevisiae</span> Chromatin Remodeler Fun30 Regulates DNA End Resection and Checkpoint Deactivation
AID  - 10.1128/MCB.00566-12
DP  - 2012 Nov 15
TA  - Molecular and Cellular Biology
PG  - 4727--4740
VI  - 32
IP  - 22
4099  - http://mcb.asm.org/content/32/22/4727.short
4100  - http://mcb.asm.org/content/32/22/4727.full
SO  - Mol. Cell. Biol.2012 Nov 15; 32
AB  - Fun30 is a Swi2/Snf2 homolog in budding yeast that has been shown to remodel chromatin both in vitro and in vivo. We report that Fun30 plays a key role in homologous recombination, by facilitating 5′-to-3′ resection of double-strand break (DSB) ends, apparently by facilitating exonuclease digestion of nucleosome-bound DNA adjacent to the DSB. Fun30 is recruited to an HO endonuclease-induced DSB and acts in both the Exo1-dependent and Sgs1-dependent resection pathways. Deletion of FUN30 slows the rate of 5′-to-3′ resection from 4 kb/h to about 1.2 kb/h. We also found that the resection rate is reduced by DNA damage-induced phosphorylation of histone H2A-S129 (γ-H2AX) and that Fun30 interacts preferentially with nucleosomes in which H2A-S129 is not phosphorylated. Fun30 is not required for later steps in homologous recombination. Like its homolog Rdh54/Tid1, Fun30 is required to allow the adaptation of DNA damage checkpoint-arrested cells with an unrepaired DSB to resume cell cycle progression.