RT Journal Article
SR Electronic
T1 Human proto-oncogene N-myc encodes nuclear proteins that bind DNA.
JF Molecular and Cellular Biology
JO Mol. Cell. Biol.
FD American Society for Microbiology
SP 4450
OP 4457
DO 10.1128/MCB.6.12.4450
VO 6
IS 12
A1 Ramsay, G
A1 Stanton, L
A1 Schwab, M
A1 Bishop, J M
YR 1986
UL http://mcb.asm.org/content/6/12/4450.abstract
AB N-myc is a gene whose amplification has been implicated in the genesis of several malignant human tumors. We have identified two proteins with molecular weights of 65,000 and 67,000 encoded by N-myc. The abundance of these proteins in tumor cells was consonant with the extent of amplification of N-myc. The two proteins apparently arose from the same mRNA, were phosphorylated, were exceptionally unstable, were located in the nucleus of cells, and bound to both single- and double-stranded DNA. These properties suggest that the products of N-myc and of the related proto-oncogene c-myc may have similar biochemical functions and that N-myc may be a regulatory gene. Our findings sustain the view that inordinate expression of N-myc may contribute to the genesis of several different human tumors.