TABLE 1.

Peptides enriched >2-fold in hTrex versus hTrex-mt DNA-affinity purified samples

ProteinaPeptideaHeavy/normalb ratioProbability scorec
Annexin a7CYQSEFGRDLEK2.7:10.9986
CNBPCGESGHLAK2.1:10.9994
DCDLQEDACYNCGR2.6:10.9997
GFQFVSSSLPDICYR2.9:10.9999
CYSCGEFGHIQK7:10.9996
CGESGHLAR9.2:11.0000
CGETGHVAINCSK10.7:10.9998
Six4YVLDGMVDTVCEDLETDKK2.4:10.9916
  • a Peptides were analyzed by μLC-ESI-MS/MS, and protein identifications were assigned using the SEQUEST algorithm to search a human protein sequence database. ICAT-labeled cysteine residues are indicated in bold.

  • b The relative abundance of each peptide in heavy (isolated from hTrex beads) versus normal (isolated from hTrex-mt beads) ICAT-labeled samples was calculated using XPRESS and is expressed as a ratio. For CNBP, the three peptides with ratios of 2.1 to 2.9 were derived from the N-terminal portion of CNBP, whereas the three peptides with ratios of 7 to 10.7 were derived from the C-terminal portion of CNBP.

  • c PeptideProphet probability scores are shown for each peptide.