TABLE 3.

Effect of the rad50, rad51, and rad59 mutations on the frequency of BIRa at other loci

Relevant genotypeFrequency of BIR at:
CFV/D8B-tgCFV/MRC1-tgCFV/YBR235-tgCFV/PCA1-tg
RAD20 ± 1039 ± 196.3 ± 2.412.8 ± 9.4
rad510.14 ± 0.050.95 ± 0.10.19 ± 0.050.36 ± 0.2
rad5011 ± 0.476 ± 198.9 ± 6.131 ± 23
rad5912 ± 7.727 ± 155.0 ± 0.717 ± 5.3
  • a The frequency of BIR for each vector is the number of stable Ura+ transformants per microgram with cut DNA divided by the number of transformants per microgram with uncut DNA transformed (10−2).