TABLE 2.

Effect of rad52 group mutations on the frequency of BIRa

Relevant genotypeFrequency of stable Ura+ (10−2) with CFV/D8B-tgFrequency relative to wild type
RAD20 ± 10100
rad52<0.0027<0.014
rad510.14 ± 0.050.70
rad540.20 ± 0.11.0
rad550.47 ± 0.32.4
rad570.52 ± 0.22.6
rad5912 ± 7.760
rad51 rad590.07 ± 0.030.35
rad5011 ± 0.455
rad50 rad510.47 ± 0.292.4
rad50 rad593.5 ± 1.618
rad50 rad51 rad590.34 ± 0.161.7
RAD/RAD17 ± 5.3100
rad51/rad510.17 ± 0.041
  • a The frequency of BIR is the number of stable Ura+ transformants per microgram with cut DNA divided by the number of transformants per microgram with uncut DNA transformed.