TABLE 2.

Plasmids used in this study

PlasmidInsertion aPlasmid used for derivationSource or reference
pSLR4 rad62 genomicpUC118This study
pSLR4D rad62::ura4 pSLR4This study
pREP41 ars1 LEU2 nmt1* 31
pREP41X ars1 LEU2 nmt1* 12
pBRC1 ars1 LEU2 nmt1* brc1 cDNApREP41XM. O'Connell
pRAD62 ars1 LEU2 nmt1* rad62 cDNApREP41This study
pRAD60 ars1 LEU2 nmt1* rad60 cDNApREP41This study
pREP41EGFP-N ars1 LEU2 nmt1* EGFPpREP41X 9
pGFP62 ars1 LEU2 nmt1* EGFP-rad62 cDNApREP41EGFP-NThis study
  • a *, nmt medium-strength promoter.