TABLE 3.

Primers used for site-directed mutagenesis of NIP1

PrimerSequence (5′ to 3′)
LVNIP1-AVAIGCC TGC CAA GCC CGA GAT CTA
LVNIP1MUTGCC AAG CCC GAG ATC TAC GCA TAT G
LV22CAT TTT CTC TAG AAA CTT
LVBOX1CCA AGC CCG AGA TCT ACG CAT ATG GCA GCT GCT GCA GCA GCA GCA GCA GCA TAC GAT GTA GCC AGT TCT TCA TCC GAA GAA
LVBOX2CCA AGC CCG AGA TCT ACG CAT ATG TCC CGT TTC TTT TCG TCT AAT TAC GAA GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA
LVBOX2CGCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAT CTT TTA TCT TCG TCT GAA
LVRBOX3BTTC TTC GGA TGA AGA ACT GGC
LVBOX3CGCC AGT TCT TCA TCC GAA GAA GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TTA AGC TCT TCC TCC TCT GAG
LVRBOX4BCAA ATC TTC TTC AGA CGA AGA
LVBOX4CTCT TCG TCT GAA GAA GAT TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAC CAA GAA TCT GAC GAC TCC
LVRBOX5BCAA TTC AGA CTC AGA GGA GGA
LVBOX5CTCC TCC TCT GAG TCT GAA TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAA AGT GAA AGT GAA AGT GAA
LVRBOX6BATT GAA AAA GGA GTC GTC AGA
LVBOX6CTCT GAC GAC TCC TTT TTC AAT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAC TCT GAT GAT TCT GAT GCA
LVNBOX61-RTCT GAC GAC TCC TTT TTC AAT CGT CGA CGA CGA CGA CGA CGA CGA CGA GTA GAC TCT GAT GAT TCT GAT
LVNBOXA2ATT GAA AAA GGA GTC GTC AGA
LVRBOX7BTAC ATC AGC TTC ACT TTC ACT
LVBOX7CAGT GAA AGT GAA GCT GAT GTA GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GGT CCT GAC TGG TTC AAG AAA
LVRBOX8BATA AGG CTT TGC ATC AGA ATC
LVBOX8CGAT TCT GAT GCA AAG CCT TAT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA AGA AAA CAA GGT GGA GGT TCA
LVRBOX9BGAA CTC AGA TTT CTT GAA CCA
LVBOX9CTGG TTC AAG AAA TCT GAG TTC GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TTG AAA AGC TCT AAC TAT GAT
LVRBOX10BAAA TTT ATT TGA ACC TCC ACC
LVBOX10CGGT GGA GGT TCA AAT AAA TTT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAA GAA TCC GAT GAA GAA GAT
LVRBOX11BATC ACT GGA ATC ATA GTT AGA
LVBOX11CTCT AAC TAT GAT TCC AGT GAT GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GTA GTC AAG TCT GCC AAA GAA
LVRBOX12BCTT CTT GCC ATC TTC TTC ATC
LVBOX12CGAT GAA GAA GAT GGC AAG AAG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA GAT GAA ATG CAA GAC GTT TAT
LVRBOX13BCAA TAG TTT TTC TTT GGC AGA
LVBOX13CTCT GCC AAA GAA AAA CTA TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TCT CAA GCT GAG AAC TCT GAT
LVRBOX14BGAT CTT ATT ATA AAC GTC TTG
LVBOX14CCAA GAC GTT TAT AAT AAG ATC GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA ACT ATT TCT AAT GAG TTT GAT
LVRBOX15BCAA CCA GTC ATC AGA GTT CTC
LVBOX15CGAG AAC TCT GAT GAC TGG TTG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA CGT CTC TTA GTT AGG GCT CAA
LVRBOX16BCGA GAT CAA ATC AAA CTC ATT
LVBOX16CAAT GAG TTT GAT TTG ATC TCG GCA GCT GCT GCA GCA GCA GCA GCA GCA GCA TGG GGG ACT CCA AAT ATT TTC
LV22-HIIIAAT ACA AGC TTA ACT TTC TTG ACT CTT TGC TT
LV91ATA TAG AGC TCT GAA AGG AAT GAA AAA TTA
LV92ATA TAG GTA CCT TTC GTA GAT CTC GGG CTT
LV101ATA TAG TCG ACC TAA GCA GGA GAG TAT AA
LV102ATA TAG CAT GCT GCA ACT GTT GTA TCT TG