TABLE 2.

Plasmids used in this study

PlasmidDescriptionSource or reference
YCpNIP1-His-USingle-copy NIP1-His, URA3 plasmid from YCplac33 29
YEpNIP1-HisHigh-copy-number NIP1-His, LEU2 plasmid from YEplac181 29
YCpNIP1-His-LSingle-copy NIP1-His, LEU2 plasmid from YCplac11This study
YCpNIP1-Box1 to YCpNIP1-Box16-HisSingle-copy NIP1-His containing 10 Ala substitutions in Box1 to Box16, respectively, LEU2 plasmid from YCplac11This study
YCpNIP1-Box6R-HisSingle-copy NIP1-His containing nine Arg substitutions in Box6, LEU2 plasmid from YCplac11This study
YEpNIP1-Box1-His to YEpNIP1-Box16-HisHigh-copy-number NIP1-His containing 10 Ala substitutions in Box1 to Box16, respectively, LEU2 plasmid from YEplac181This study
YEpNIP1-Box6R-HisHigh-copy-number NIP1-His containing nine Arg substitutions in Box6, LEU2 plasmid from YEplac181This study
YEpNIP1-N′-His-XHigh-copy-number NIP1-N′-His [1-205], LEU2 plasmid from YEplac181This study
YEpNIP1-N′-Box2, -4, -6, -12, -14, and -15-HisHigh-copy-number NIP1-N′-His[1-205] containing 10 Ala substitutions in Box2, -4, -6, -12, -14, and -15, respectively, LEU2 plasmid from YEplac181This study
YEpNIP1-N′-Box6R-HisHigh-copy-number NIP1-N′-His[1-205] containing Box6R (9-Arg substitutions), LEU2 plasmid from YEplac181This study
pT7-NIP1-N′ NIP1[1-205] ORF under T7 promoterThis study
pT7-NIP1-N′-Box2, -4, -12, -14, and -15 NIP1[1-205] ORF containing ten Ala substitutions in Box2, -4, -12, -14, and -15, respectively, under T7 promoterThis study
pT7-NIP1-N′-Box6R NIP1[1-205] ORF containing nine Arg substitutions in Box6 under T7 promoterThis study
pLV10 nip1Δ::hisG::URA3::hisG This study
YEpTIF32-Δ6-His-UHigh-copy-number TIF325-His[1-790], LEU2 plasmid from YEplac181 29
YEpTIF5-UHigh-copy-number TIF5-FLAG, URA3 plasmid from YEplac195This study
YEpTIF5-7A-UHigh-copy-number tif5-7A-FLAG, URA3 plasmid from YEplac195This study
YEpSUI1-UHigh-copy-number SUI1, URA3 plasmid from YEplac195This study
YEpTIF5+SUI1High-copy-number TIF5-FLAG and SUI1, URA3 plasmid from YEplac195 29
pRSSUI3-S264Y-ULow-copy SUI3-S264Y, URA3 plasmid from pRS316This study
YCpTIF5-G31R-USingle-copy TIF5-G31R, URA3 plasmid from YCplac33This study
YCpSUI3-S264Y-WSingle-copy SUI3-S264Y, TRP1 plasmid from YCplac22This study
YCpTIF5-G31R-WSingle-copy TIF5-G31R, TRP1 plasmid from YCplac22This study
p1780-IMTHigh-copy-number SUI2, SUI3, GCD11, IMT4, URA3 plasmid from YEp24 3
pGEX-TIF5 GST-TIF5 fusion plasmid from pGEX-4T-1 26
pGEX-SUI1 GST-SUI1 fusion plasmid from pGEX-5X-3K. Asano
pGEX-sui1-1 GST-SUI1-D83G fusion plasmid from pGEX-5X-3K. Asano
p367Low-copy-number URA3 vector containing HIS4-ATG-lacZ fusion 5
p391Low-copy-number URA3 vector containing HIS4-TTG-lacZ fusion 5
p2042Low-copy-number URA3 vector containing HIS4-ATT-lacZ fusion, third codon replaced with TTA 14
p180Low-copy-number URA3 vector containing GCN4-lacZ fusion 20
YCplac22Single-copy cloning vector, TRP1 9
YCplac33Single-copy cloning vector, URA3 9
YEplac112High-copy-number cloning vector, TRP1 9
YEplac195High-copy-number cloning vector, URA3 9