Table 1.

Repair efficiency and distribution of Ura+ products

Relevant genotypeRepair efficiencyaNo. of Ura+ coloniesb
Total analyzedNoncrossoverCrossoverMixedcAberrantd% Crossoversb
3-day plate induction
RAD1 RAD51 77.3 ± 9.350 (58)36 (44)1 (9)852 (16)
rad1 RAD51 74.2 ± 8.150 (67)25 (42)3 (20)1746 (30)
RAD1 rad51 50.4 ± 8.334 (37)19 (22)9 (12)3326 (32)
rad1 rad51 21.4 ± 7.352 (60)14 (22)29 (37)8155 (62)
24-h liquid induction
RAD1 RAD51 54.318 17 1 006 
rad1 RAD51 54.518 (19)15 (16)1 (2)116 (11)
RAD1 rad51 25.818 12 6 0033 
rad1 rad51 10.818 (19)6 9 (10)1250 (53)
  • a Percentage of Ura+ colonies after HO induction (see Materials and Methods).

  • b Numbers in parentheses count the mixed populations as two one-crossover and one noncrossover product.

  • c Mixed population of crossover and noncrossover products.

  • d Plasmids with restriction fragments inconsistent with either crossover or noncrossover products.