Primers used in this study
| Gene | Primer sequencea | Reference |
|---|---|---|
| Xbra | F, GGATCGTTATCACCTCTG | 73 |
| R, GTGTAGTCTGTAGCAGCA | ||
| Ef1α | F, CAGATTGGTGCTGGATATGC | 73 |
| R, ACTGCCTTGATGACTCCTAG | ||
| ODC | F, CAGCTAGCTGTGGTGTGG | 1 |
| R, CAACATGGAAACTCACACC | ||
| Sox17β | F, AACTCCCACCAGCAGGCTACTTTG | |
| R, TGTCAATGTCACTCTCCAGATGTCC | ||
| Gsc | F, ACAACTGGAAGCACTGGA | 73 |
| R, TCTTATTCCAGAGGAACC | ||
| Endodermin | F, TATTCTGACTCCTGAAGGTG | 57 |
| R, GAGAACTGCCCATGTGCCTC | ||
| NCAM | F, CACAGTTCCACCAAATGC | 31 |
| R, GGAATCAAGCGGTACAGA | ||
| Sef | F, GTCGAATTCGCCTGCAACGACCAAGTGGC | |
| R, CTGCGAATTCGCCCTCGTGCTTCAGTTTG | ||
| Sprouty2 | F, CATTCCTTGTTTCAGGC | |
| R, GATGGGAGAGTCCTTGG |
↵a F, forward; R, reverse.