TABLE 1.

S. cerevisiae strains used in this study

StrainGenotypeSource
SH777 MAT a/MATα ade2/ade2 can1:ADE2:CAN1/can1:ADE2:CAN1 his3-11,15/his3-11,15 leu2-3,112/ leu2-3,112 lys2 (3′Δ):HIS3:lys2(5′Δ)/LYS2 trp1-1/trp1-3′Δ ura3-1/ura3-1 32
SH1740SH777 except grr1Δ::TRP1/grr1Δ::TRP1 This study
SH1970SH777 except TRP1/TRP1 LEU2/LEU2 38
SH1752SH777 except grr1Δ::TRP1/grr1Δ::TRP1 LEU2/LEU2 38
SH2778SH777 except trp1/TRP1 This study
SH2716 MATα ade2 can1:ADE2:CAN1 his3-11,15 leu2-3,112 trp1-3′Δ ura3-1 grr1::URA3 w/GAL1-IME2::6XHA-TRP1 S. Irniger
SH2811SH1970 containing pS652 (tetO-IME2-6XHA-URA3)This study
SH2813SH1752 containing pS652 (tetO-IME2-6XHA-URA3)This study
SH2877SH1970 containing pS669 [tetO-IME2(pestΔ)-6XHA-URA3]This study
SH3219SH1740 containing pS714 (tetO-IME2-6XHA-LEU2) and pTer62 (cup1-mycUb.Ko-URA3)This study
SH3204SH2778 containing pRS315 (LEU2) and pTer62 (Cup1-mycUb.Ko-URA3)This study
SH2362SH1970 containing pRS316 (URA3)This study
SH3195SH2778 containing pS714 (tetO-IME2-6XHA-LEU2) and pTer62 (Cup1-mycUb.Ko-URA3)This study
W303 MAT a/MATα ade2/ade2 can1/can1 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1
MTY1537W303 except cdc34-2/cdc34-2 M. Tyers
MTY1538W303 except cdc53-1/cdc53-1 M. Tyers
MTY1831 MAT a ade2 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 sic1ΔM. Tyers
SH3179MTY1537 with pS652 (tetO-IME2-6XHA-URA3)This study
SH3181MTY1538 with pS652 (tetO-IME2-6XHA-URA3)This study
SH3183W303 with pS652 (tetO-IME2-6XHA-URA3)This study
SH2898SH777 except grr1Δ::TRP1/GRR1 LEU2/leu2-3,112 This study
SH2896SH777 except grr1Δ::TRP1/grr1Δ::TRP1 LEU2/leu2-3,112 This study