TABLE 1.

S. cerevisiae strains used for this study

StrainGenotypeReference or source
By4741 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Open Biosystems
YKL101 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:Ada2-TAP::HIS3 MX6 Open Biosystems
YKL134 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:Ada2TAP::HIS3 MX6 ubp8Δ::KANMX6 This study
YKL128 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:Ada2-TAP::HIS3 MX6 sgf11Δ::KANMX6 This study
YKL117 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:Ubp8-TAP::HIS3 MX6 Open Biosystems
YKL132 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:Ubp8-TAP::HIS3 MX6 sgf11Δ::KANMX6This study
YKL138 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:Sgf11-TAP::HIS3 MX6 ubp8Δ::KANMX6This study
YKL60 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:gen5Δ::KANMX6Open Biosystems
YKL120 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:ubp8Δ::KANMX6Open Biosystems
YKL97 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:sgf11Δ::KANMX6Open Biosystems
YKL136 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:gen5Δ::KANMX6 ubp8Δ::KANMX6This study
YKL116 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:gen5Δ::KANMX6 sgf11Δ::KANMX6This study
YKL137 mat a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0:ubp8Δ::KANMX6 sgf11Δ::KANMX6This study
YKH045 MAT a ura3-1 leu2,3,-112 his3-11,-15 trp1-1 ade2-1 htb1-1 htb2-1 pRS314 (Flag-HTB1-CEN-TRP1) pRG145 (GAPDHprom-3HA-UB14-URA3 integrative) 10
YKH046 MAT a ura3-1 leu2,3,-112 his3-11,-15 trp1-1 ade2-1 htb1-1 htb2-1 pRS314 (Flag-htb1K123R-CEN-TRP1) pRG145 (GAPDHprom-3HA-UB14-URA3 integrative) 10
YKH047 MAT a ura3-1 leu2,3,-112 his3-11,-15 trp1-1 ade2-1 htb1-1 htb2-1 pRS314 (Flag-HTB1-CEN-TRP1) ubp8Δ::KanMx pRG145 (GAPDHprom-3HA-UB14-URA3 integrative) 10
YKL142 MAT a ura3-1 leu2,3,-112 his3-11,-15 trp1-1 ade2-1 htb1-1 htb2-1 pRS314 (Flag-HTB1-CEN-TRP1) sgf11Δ::LEU2 pRG145 (GAPDHprom-3HA-UB14-URA3 integrative)This study
YKL143 MAT a ura3-1 leu2,3,-112 his3-11,-15 trp1-1 ade2-1 htb1-1 htb2-1 pRS314 (Flag-HTB1-CEN-TRP1) gen5Δ::LEU2 pRG145 (GAPDHprom-3HA-UB14-URA3 integrative)This study
Fy2034 MAT a HA-SPT7-TAP::TRP1 ura3Δ0 leu2Δ1 his3Δ200 gen5::HIS3 trp1Δ63 lys2-173R2 33
YJW589 MAT a HA-SPT-TAP::TRP1 ura3Δ0 leu2Δ1 his3Δ200 gen5DBr::KANMX6 trp1Δ63 lys2-173R2 Mark Chandy