TABLE 2.

Genotypes of offspring from intercrosses of Pcyt1a+/− mice

Age aNo. with genotype No. resorbedbTotal
+/++/−−/−
E3.5122210NA44
    Blastocystc 9210NA30
    Morulac 3110d NA14
E8.57170024
E11.519410060
3 wks40800NA120
  • a Animals from intercrosses of CCTα+/− mice were collected at E3.5, E11.5, or 3 weeks after birth, and the genotypes were determined by PCR or PCR followed by Southern blotting as described in Materials and Methods.

  • b The uterus was examined for evidence of resorbed embryos. NA, not applicable.

  • c Genotypes were determined by PCR analysis followed by Southern blotting as described in Materials and Methods.

  • d The Pcyt1a −/− embryos did not have normal morphology.