TABLE 1.

Real-time PCR primer-probe sets

PrimerSequenceProbe a
mCCTα-Forward5′-GATGAGCTAACGCACAACTTCAA 6FAM-CGCTCATTCTCGTTCATCACAGTGA-TAMRA
mCCTα-Reverse5′-GTGCTGCACGGCGTCATA
mCCTβ2-Forward5′-TTCTTTGCCTGGGAGGAGACT 6FAM-TGCTCCCTCCAGCTCTACACCCT-TAMRA
mCCTβ2-Reverse5′-AAGTACTGGCATGGCCAGTGA
mCCTβ3-Forward5′-GGGCCAAACCTTGTGGTACA 6FAM-AATTCGTCCTTGTCCATGCTGCAT-TAMRA
mCCTβ3-Reverse5′-TGCAGTCAGGGTCTTGCGT
mPEMT-Forward5′-GGCATCTGCATCCTGCTTTT 6FAM-CTCCGCTCCCACTGCTTCACAC-TAMRA
mPEMT-Reverse5′-TTGGGCTGGCTCACTATAGC
  • a 6FAM, 6-carboxyfluorescein; TAMRA, tetramethyl carboxy rhodamine.