TABLE 1.

Luciferase activitiesa

ConstructAdriamycinLuciferase activity (RLU/μg of extract)SDFold repression
Cyclin B2-luciferase5,715437
Cyclin B2-luciferase+2,2648982.6
CycB-Y1-Y2m1623
CycB-Y1-Y2m+135210.2
  • a NIH 3T3 cells stably transfected with the indicated luciferase constructs were not treated (−) or treated (+) with adriamycin for 8 h and assayed for luciferase activities, normalized for the protein concentrations of the extracts. The basal levels of the pGL2Basic transfection vector are 5 to 10 relative light units (RLU)/μg of extract.