TABLE 2.

DNA and histone modifications of the HRD transgene

Cell or tissue transgene modificationModification retention in indicated straina
B6D2
Undifferentiated ES cells
    Inactivating
        DNA methylation (meCpG)+/−
        Histone H3 lysine-9 methylation (H3-K9me)+/−+/−
    Activating
        Histone H3 lysine-4 methylation (H3-K4me)+/−+/−
        Histone H3 acetylation (K9ac and K14ac)+/−+/−
        Brg1 (SWI/SNF) binding+/−+/−
Liver
    Inactivating
        DNA methylation (meCpG)+
        Histone H3 lysine-9 methylation (H3-K9me)+
        MeCP2 binding+
    Activating
        Histone H3 lysine-4 methylation (H3-K4me)++
        Histone H3 acetylation (K9ac and K14ac)+
        Histone H3 acetylation (K14ac)+
        Brg1 (SWI/SNF) binding+
  • a +, strong modification; −, weak or no modification; +/−, intermediate modification.