TABLE 1.

Bisulfite sequencing of the GPT region of the HRD transgene

GPT region of strain or plasmid% of total CpGs not converteda% of CpA, CpC, and CpT conversionb
B6
    Undifferentiated
        First half4589
        Second half2689
    Adult entire GPT region9387
D2
    Undifferentiated
        First half1591
        Second half1492
    Adult entire GPT region1091
Plasmid
    First half891
    Second half994
  • a The percentages of total CpGs not converted and thus methylated.

  • b The percentages of conversion of CpA, CpC, and CpT as a measure of the efficiency of the bisulfite reaction.