TABLE 1.

Genes that are differentially expressed in the absence of EKLF

GeneaSymbolGene expressionb in:
Cultured cellsFetal liver
T = 0T = 12
Downregulated in EKLF KO
    Heme synthesis
        • Aminolevulinic acid synthase 1Alas10.10.20.2
        • Hydroxymethylbilane synthase/porphobilinogen deaminaseHmbs/PBGD0.20.51.1
        • Uroporphyrinogen decarboxylaseUrod−0.10.30.6
        • Coproporphyrinogen oxidaseCpox0.0−0.30.0
        • Protoporphyrinogen oxidasePpox0.70.30.8
        • FerrochelataseFech−0.20.00.6
    Erythroid cell specific
        • Hemoglobin, beta adult major chainHbb-b10.90.83.5
        ▴ Hemoglobin, beta adult major chainHbb-b10.70.53.0
        • Hemoglobin Z, beta-like embryonic chainHbb-bh10.70.31.6
        • HemogenHemgn−0.20.61.5
        ▴ Alpha hemoglobin stabilizing proteinEraf1.02.53.6
    Cell membrane
        • Erythrocyte protein band 4.9Epb4.90.40.53.7
        • K+ intermediate/small conductance Ca2+-activated channel, subfamily N, member 4Kcnn40.81.02.5
        ▴ Solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2Slc3a20.21.22.9
        • Solute carrier family 2 (facilitated glucose transporter), member 1Slc2a1−0.2−0.61.5
        • Solute carrier family 22 (organic cation transporter), member 4Slc22a40.20.31.7
        • Solute carrier family 43, member 1Slc43a10.50.71.8
        • Kell blood groupKel−0.10.41.9
        • CD24a antigenCd24a0.30.31.5
        • Transferrin receptorTfrc0.20.82.0
        • Intercellular adhesion molecule 4, Landsteiner-Wiener blood groupIcam4−0.10.01.7
    Other
        • Protease, serine, 25Prss250.91.04.7
        • Sin3 associated polypeptideSap300.20.82.1
        • Interferon-induced protein with tetratricopeptide repeats 3Ifit30.40.42.1
        • Ubiquitin associated domain containing 1Ubadc10.40.41.6
        • unc-84 homolog A (C. elegans)Unc84a0.80.62.2
        • RIKEN cDNA 1110063G11 gene1110063G11Rik0.10.81.9
        • RIKEN cDNA 1200006I17 gene1200006I17Rik−0.40.11.8
        • Monoglyceride lipaseMgII0.00.31.4
        • Cat eye syndrome chromosome region, candidate 2 homolog (human)Cecr21.00.71.8
        • Lectin, galactose binding, soluble 1Lgals10.71.01.5
        • Myeloid differentiation primary response gene 116Myd1160.30.21.4
        • Guanine nucleotide binding protein, alpha inhibiting 2Gnai21.20.94.0
        ▴ Guanine nucleotide binding protein, alpha inhibiting 2Gnai21.00.93.2
        ▴ Mitogen-activated protein kinase kinase 5Map2k50.70.73.1
        ▴ Hypothetical guanylate-kinase-associated proteinDlg71.21.03.6
        ▴ CCR4-NOT transcription complex, subunit 7Cnot70.40.44.0
        • Mus musculus RIKEN cDNA 2700084L06 gene2700084L06Rik0.71.01.3
        • Peroxiredoxin 2Prdx20.40.61.2
        • Microsomal glutathione S-transferase 3Mgst30.00.31.9
        • Nuclear factor of kappa light chain gene enhancer in B-cell inhibitor, alphaNfkbia0.61.01.6
        • Glutathione peroxidase 4Gpx41.4
Upregulated in EKLF KO
    • Coagulation factor II (thrombin) receptorF2r−1.0−0.4−1.3
    • Enolase 1, alpha non-neuronEno10.0−0.4−1.2
    • Heme oxygenase (decycling) 2Hmox20.0−0.2−2.9
    • Phosphoglycerate mutase 1Pgam1−0.1−0.1−1.2
    • RIKEN cDNA 1110014J01 gene1110014J01Rik−0.30.0−2.3
    • Kelch domain containing 3Klhdc30.1−0.1−1.8
    • 24-Dehydrocholesterol reductaseDhcr24−0.30.3−1.9
    • RIKEN cDNA 6720456B07 gene6720456B07Rik0.00.1−2.1
    • Thrombospondin 1Thbs10.1−0.4−2.5
    • Guanosine monophosphate reductaseGmpr−0.7−0.2−1.6
    • Ethanolamine kinase 1Etnk1−0.7−0.5−1.6
    • Metastasis suppressor 1Mtss10.2−0.1−1.6
    ▴ Carbonic anhydrase 1Car1−1.2−1.3−2.5
    ▴ Pyruvate kinase, musclePkm2−1.6−1.1−1.7
  • a KO, knockout; • and ▴, from the 17K and erythroid cell-enriched 9K microarrays, respectively.

  • b Gene expression in wild-type and EKLF KO cultured cells at 0 and 12h after differentiation and in wild-type and EKLF KO fetal livers was determined with cDNA microarrays. Values are the 2log of the wild-type/EKLF KO ratio. The average of values was taken when genes were represented more than once on the chips. Genes with 2log ratios higher than 1.2 or lower than −1.2 in fetal livers and genes involved in heme synthesis are displayed.