TABLE 1.

Oligonucleotide primers and sizes of PCR products for competitive RT-PCR

Gene productPrimeraSize of PCR products (bp)Restriction enzyme(s) for construction of competitor DNA
TargetCompetitor
Insulin 15′-CCAGCTATAATCAGAGACCA-3′377328SmaI-BsgI
5′-GGGCCTTAGTTGCAGTAGTT-3′
Insulin 25′-AGGAAGCCTATCTTCCAGGT-3′401293EcoNI-SmaI
5′-ATTCATTGCAGAGGGGTAGG-3′
Glucagon5′-CTGGACAATCTTGCCACCAGGGAC-3′413358SphI-BstXI
5′-CCACTACGGTTACCAGGTGGTCATGTC-3′
Pdx15′-TCGCTGGGATCACTGGAGCA-3′365247BlpI-MluI
5′-CGGGAGATGTATTTGTTAAATAAGAATTC-3′
Beta25′-CTCCAGGGTTATGAGATCGTCAC-3′525329PpuMI
5′-GATCTCTGACAGAGCCCA-3′
Glut-25′-TGGGATGAAGAGGAGACTGAA-3′652442XcmI-BsrGI
5′-CATCCGTGAAGAGCTGGATCA-3′
Glucokinase5′-GATGTATTCCATCCCCGAGGACG-3′410291StyI-MscI
5′-GCTCCACATTCTGCATCTCCTCC-3′
HPRT5′-GGCTTCCTCCTCAGACCGCTTT-3′702523BclI-PpuMI
5′-AGGCTTTGTATTTGGCTTTTCC-3′
  • a The sequence of the forward primer is given on the top, and that of the reverse primer is given on the bottom.