TABLE 1.

S. pombe strains used in this study

StrainGenotypeaSource or reference
PR109Wild typeLab stock
CC3223nbs1::kanMX611
LLD3260h leu1-32::2YFP-crb2+-leu1+ ura4-D18 crb2-D2::ura4+22
LLD3341his3-D1 crb2::ura4+-2YFP-crb2+-leu1+ tel1-D1::kanMX4 rad3::ura4+51
CC3575nbs1+-TAP:kanMX6This study
CC3576nbs1ΔC60-TAP:kanMX6This study
CC3577nbs1-9-TAP:kanMX6This study
CC3578nbs1-10-TAP:kanMX6This study
CC3579kanMX6:nmt1-TAP-tel1+ nbs1+-13myc:kanMX6This study
CC3580kanMX6:nmt1-TAP-tel1+ nbs1-9-13myc:kanMX6This study
CC3581kanMX6:nmt1-TAP-tel1+ nbs1-10-13myc:kanMX6This study
CC3582rad3::ura4+ nbs1+ -TAP:kanMX6 mrc1+-TAP:kanMX6This study
CC3583rad3::ura4+ nbs1 ΔC60-TAP:kanMX6 mrc1+-TAP:kanMX6This study
CC3584rad3::ura4+ nbs1-9-TAP:kanMX6 mrc1+-TAP:kanMX6This study
CC3585rad3::ura4+ nbs1-10-TAP:kanMX6 mrc1+-TAP:kanMX6This study
CC3586rad3::ura4+ tel1-D2:Leu2 mrc1+-TAP:kanMX6This study
CC3587ade6 his3-D1 crb2::ura4+ -2YFP-crb2+-leu1+ rad3::ura4+ nbs1+-TAP:kanMX6This study
CC3588ade6 his3-D1 crb2::ura4+-2YFP-crb2+-leu1+ rad3::ura4+ nbs1ΔC60-TAP:kanMX6This study
CC3589ade6 his3-D1 crb2::ura4+-2YFP-crb2+-leu1+ rad3::ura4+ nbs1-9-TAP:kanMX6This study
CC3590ade6 his3-D1 crb2::ura4+-2YFP-crb2+-leu1+ rad3::ura4+ nbs1-10-TAP:kanMX6This study
  • a All strains are h leu1-32 ura4-D18.