TABLE 1

Primary antibodies used in this studya

AntibodyTypeDilutionSource
IFWB
ADAM10PolyclonalNA1:2,000EMD Millipore Co.
APH1PolyclonalNA1:800G. Thinakaran
APP (clone Y188)Monoclonal1:1,0001:5,000Abcam Inc.
APP (clone 22C11)Monoclonal1:2,000NAEMD Millipore Co.
APP NTG-449PolyclonalNA1:1,000G. Thinakaran
β-ActinMonoclonalNA1:5,000Sigma-Aldrich, Inc.
β-Amyloid, 17–24 (4G8)Monoclonal1:1,000NACovance Corp.
β-Amyloid, 1–16 (6E10)MonoclonalNA1:3,000Covance Corp.
BACE1PolyclonalNA1:2,000Abcam Inc.
GAPDHMonoclonalNA1:3,000Sigma-Aldrich, Inc
LAMP1Polyclonal1:500NAAbcam Inc.
LC3Polyclonal1:1,0001:1,000MBL International Corp.
NicastrinPolyclonalNA1:500Santa Cruz Biotechnology, Inc.
Pen2PolyclonalNA1:2,000G. Thinakaran
Presenilin1-NTF (PS1-NTF)PolyclonalNA1:3,000G. Thinakaran
sAPPα (2B3)MonoclonalNA1:1,000IBL Co., Ltd.
sAPPβ-wtPolyclonalNA1:1,000IBL Co., Ltd.
sAPPβ-sw (6A1)MonoclonalNA1:3,000IBL Co., Ltd.
SREBP2PolyclonalNA1:500Abcam Inc.
  • a IF, immunofluorescence; WB, Western blotting; NA, not used in that specific application.