TABLE 3.

Zebra fish gata-1 promoter-dependent EGFP expression in the presence of zMelk-like gene or gata-1

Material(s) injectedNo. of embryos examinedaNo. of EGFP-positive embryos (%)
No expressionStrong levelWeak levelEctopicLethal
gata-1 promoter EGFP(50 pg)653 (4%)5 (7%)53 (83%)2 (3%)2 (3%)
gata-1 promoter EGFP(100 pg) + zMelk-like gene (300 pg)724 (6%)33 (45%)28 (39%)3 (4%)4 (6%)
gata-1 promoter EGFP(100 pg) + gata-1 (200 pg)565 (9%)33 (60%)7 (12%)36 (65%)8 (14%)
  • a Expression levels were arbitrary examined under a fluorescence dissection microscope. Typical examples of strong and weak levels are shown in Fig. 5B.