TABLE 1.

Frequency of cell populations in lymphoid organs of ISG15+/+ and ISG15−/− mice

Tissue typeCell typeSurface marker% Cellsa
ISG15+/+ISG15−/−
ThymusDouble-positive thymocytesCD4+ CD8+ 71.7 ± 1.976.7 ± 1.4
CD4+ thymocytesCD4+ CD8 14.4 ± 0.812.3 ± 0.8
CD8+ thymocytesCD4 CD8+ 4.6 ± 0.34.1 ± 0.4
Double-negative thymocytesCD4 CD8 9.4 ± 1.26.9 ± 0.9
Bone marrowBlast cells (progenitor and stem cells)CD31high Ly6-Clow 2.4 ± 0.12.4 ± 0.1
Lymphoid cellsCD31intermediate Ly6-Clow 14.1 ± 0.614.9 ± 0.5
Erythroid progenitor cellsCD31low Ly6-Clow 4.0 ± 0.54.6 ± 0.2
Myeloid progenitor cellsCD31positive Ly6-Cpositive 13.6 ± 0.313.8 ± 0.3
GranulocytesCD31low Ly6-Cintermediate 40.9 ± 0.840.7 ± 0.6
MonocytesCD31low Ly6-Chigh 18.9 ± 1.317.4 ± 0.5
SpleenCD4+ T cellsCD3+ CD4+ 14.7 ± 0.615.0 ± 0.7
CD8+ T cellsCD3+ CD8+ 10.3 ± 0.613.9 ± 1.0
Immature B cellsB220+ IgMhigh IgDlow 13.1 ± 0.814.7 ± 0.5
Mature B cellsB220+ IgMlow IgDhigh 33.7 ± 2.827.2 ± 3.9
GranulocytesGr1high F4/80low 1.2 ± 0.31.4 ± 0.4
MonocytesCD11blow F4/80low 4.4 ± 0.25.8 ± 0.5
EosinophilsCD11b F4/80intermediate 2.6 ± 0.23.3 ± 0.2
Lymph nodeCD4+ T cellsCD3+ CD4+ 33.1 ± 1.338.6 ± 1.1
CD8+ T cellsCD3+ CD8+ 32.4 ± 1.432.2 ± 1.6
B cellsB220+ IgM+ 19.0 ± 1.617.2 ± 1.7
GranulocytesGr1high F4/80low 0.1 ± 0.00.1 ± 0.0
MonocytesCD11blow F4/80low 1.5 ± 0.31.1 ± 0.3
EosinophilsCD11b F4/80intermediate 0.3 ± 0.00.3 ± 0.0
  • a Mean percentage ± SEM is given for groups of 5 to 12 animals analyzed in two or more independent experiments.