Average identity length at junction breakpoints depends on the rearrangement formed and the strain genotype

Rearrangement classSubtype or geneNo. of rearrangementsAvg identity (range)Theoretical avg (range)a
All3583.7 (0-15)0.46 (0-21)
MonocentricInterchromosomal1223.6 (0-15)0.46 (0-21)
Isochromosome114.4 (2-10)0.46 (0-18)
Subtelomeric capture153.8 (0-13)0.44 (0-13)
Telomeric capture0NAbNAb
Interstitial deletion433.1 (0-14)0.46 (0-15)
DicentriccInterchromosomal214.3 (0-11)0.46 (0-21)
Isochromosome197.1 (1-14)0.46 (0-18)
Subtelomeric fusion423.2 (0-14)0.43 (0-13)
Telomeric fusion24NAbNAb
RepetitiveTy elements462.5 (0-10)
rRNA gene93.7 (0-8)
Complexd64.0 (0-9)
NHEJ mutationseyku70/hdf177.4 (3-14)
lig4245.8 (0-14)
HR mutationserad52212.4 (0-6)
rad54f121.8 (0-4)
rad51363.4 (0-12)
rad55153.3 (0-13)
rad59373.3 (0-13)
rdh54/tid1143.9 (0-10)
rad51 rad5972.7 (0-5)
  • a Analyzed from a computational generation of all translocations involving breakpoint regions of chromosome V and appropriate regions of the rest of the genome (over 1.3 × 1011 translocations were generated and analyzed for the monocentric interchromosomal translocations alone).

  • b NA, not applicable.

  • c Dicentric products are predicted on the basis of the sequence orientation at the breakpoint.

  • d Rearrangements involving chromosome V that would be predicted to generate direct repeats (n = 4) or circular chromosomes (n = 2).

  • e For strains containing mutations, breakpoints are included whenever the mutation occurs so that, for example, breakpoints in rad52 rad51 and rad52 rad51 rad59 strains would be included in both the rad52 and rad51 entries.

  • f Five rad54 rearrangements were from a rad54 tlc1 strain five other rad54 tlc1 isolates were telomeric fusions and are not considered here), and seven were from a rad54 rad52 strain.