TABLE 2.

EGF-induced recruitment of the EGFR to coated pits in cells transfected with siRNA to the μ2 or α subunit of AP2a

Cell transfectionEGFR-positive coated pits (%)EGFR in coated pits (%)
μ2 siRNA with EGF30.0 ± 0.57.8 ± 1.5
α siRNA with EGF17.0 ± 2.54.7 ± 1.2
  • a By immuno-EM, the number of coated pits and the plasma membrane distribution of the EGFR were quantified in nontransfected (control; see Table 1) HeLa cells and in HeLa cells transfected with siRNA to the μ2 or α subunit of AP2 upon incubation with EGF (60 ng/ml) for 1h on ice. The number of EGFR-positive coated pits is presented as the percentage of the total number of coated pits observed. The EGFR localized to coated pits is presented as the percentage of the total labeling for EGFR found at the plasma membrane. Each data set was derived from three independent labelling experiments ± SD.