TABLE 1.

EGF-induced recruitment of the EGFR to coated pits in cells transfected with EH95a

Cell cultureEGFR-positive coated pits (%)EGFR in coated pits (%)
Control without EGF1.3 ± 0.90.7 ± 0.5
Control with EGF23.0 ± 2.29.2 ± 2.7
EH95 with EGF26.7 ± 1.213.6 ± 1.4
  • a By immuno-EM, the number of coated pits and the plasma membrane distribution of the EGFR were quantified in nontransfected (control) HeLa cells and in HeLa cells transfected with EH95 and incubated with or without EGF (60 ng/ml) for 1 h on ice. The number of EGFR-positive coated pits is presented as the percentage of the total number of coated pits observed. The EGFR localized to coated pits is presented as the percentage of the total labeling for EGFR found at the plasma membrane. Each data set was derived from three independent labeling experiments ± SD.