Suppression of rec2 meiotic deficiency by Brh2-RPA70

CrossaBasidiospore viabilityb (%)Fuz+ progenyc (%)Recombination frequency (10−4)d
wt × wt45 ± 100.87.8
rec2 × rec2<10−3
rec2 × rec2/Brh2<10−3
rec2 × rec2/Brh2-RPA7015 ± 5467.6
  • a Strains were as follows: wild type (wt) × wt, UCM350 × UCM567; rec2 × rec2, UCM54 × UCM626.

  • b Teliospores were spread on YEPS plates and incubated for 5 days. Colony formation was taken as a measurement of viable basidiospore generation.

  • c A total of 200 to 300 random meiotic products obtained after germinating teliospores for 36 h and then dispersing microcolonies into single cells were spread onto medium containing charcoal. The percentage of colonies that turned white and fuzzy was determined after incubation for 3 days.

  • d Teliospores from UCM350 × UCM567 (wt × wt) and UCM54 × UCM626 (rec2 × rec2/Brh2-RPA70) were germinated on YEPS for 38 h. Microcolonies were collected and dispersed into single cells which were spread onto supplemented minimal medium containing nitrate as the sole nitrogen source to determine recombination and on YEPS to determine viability. Nar+ recombinants were identified as colonies appearing on nitrate minimal medium after 5 days.