TABLE 2.

Bas1 binding sites in sporulating yeast cells and associated meiotic recombination activitiesa

GeneORFBas1 bindingbRecombination activitycUp/downe
Wild typebas1Pd
GCV2YMR189W5.830.34230.33566.29E−01NS
MRPS17YMR188C0.29340.90861.51E−06Up
SHM2YLR058C5.470.89280.58327.98E−04Down
JEN1YKL217W5.290.61040.56265.82E−01NS
SRY1YKL218C0.28050.17891.29E−01NS
GCV3YAL044C5.150.98040.95932.32E−01NS
ADE2YOR128C5.140.39320.55584.50E−01NS
GCV1YDR019C4.970.33020.31277.51E−01NS
MTD1YKR080W4.790.71240.90181.52E−02Up
ADE17YMR120C4.710.80010.7285.12E−02Down
ADE4YMR300C4.610.26830.15911.30E−01NS
ADE12YNL220W4.540.80160.67845.18E−02Down
POP4YNL221C0.3870.37417.07E−01NS
ADE13YLR359W4.430.31880.16153.23E−03Down
HIS4YCL030C4.170.98830.70483.34E−04Down
IES5YER092W4.160.9220.97254.10E−03Up
MET6YER091C0.63640.73545.28E−01NS
ADE6YGR061C3.840.63420.65382.59E−01NS
ADE1YAR015W3.770.84390.58014.57E−03Down
ADE8YDR408C3.700.91990.85677.93E−01NS
SIZ1YDR409W0.59090.52274.37E−01NS
KTI12YKL110C3.670.59810.73456.13E−01NS
HIS1YER055C3.610.26080.45735.66E−01NS
PIR1YKL164C3.450.20070.19175.25E−01NS
PIR3YKL163W0.23420.19325.17E−01NS
CEM1YER061C3.290.04920.07438.56E−01NS
STR3YGL184C3.250.46640.25352.58E−01NS
MTO1YGL236C3.130.59680.49635.76E−01NS
ADE5,7YGL234W0.32430.36019.81E−01NS
SNO1YMR096W3.100.48660.63965.64E−01NS
SNZ1YMR095C0.45410.49175.16E−01NS
YPR004CYPR004C2.650.30170.12063.04E−01NS
MNT4YNR059W2.370.32730.21854.50E−01NS
GID8YMR135C2.230.80220.92167.44E−02NS
GAT2YMR136W0.78930.87964.85E−01NS
URA2YJL130C2.220.41020.3434.78E−01NS
SUT2YPR009W2.210.44040.08383.42E−03Down
YDL025CYDL025C2.180.09230.0521.44E−01NS
ICL1YER065C2.170.52140.55826.89E−01NS
ADE3YGR204W2.140.91190.93213.81E−01NS
ECM21YBL101C2.100.42690.35387.62E−01NS
TRP2YER090W2.090.37690.31395.13E−01NS
PTC2YER089C0.52640.47695.39E−01NS
HPT1YDR399W1.880.88010.90094.15E−01NS
ZRC1YMR243C1.820.50220.47752.09E−01NS
MCH1YDL054C1.760.24610.12232.95E−02Down
STF1YDL130W-A1.750.60780.70919.06E−02NS
RSM10YDR041W1.710.87250.91924.94E−01NS
YDR089WYDR089W1.680.9180.84923.08E−02Down
BNA6YFR047C1.590.96140.92713.50E−01NS
RBA50YDR527W1.580.34390.39755.81E−01NS
SER2YGR208W1.570.77790.84966.42E−01NS
SEC20YDR498C1.450.31980.68919.95E−04Up
LCD1YDR499W0.45360.51121.66E−01NS
MIR1YJR077C1.420.99910.99692.66E−01NS
BNA2YJR078W0.99350.98733.70E−01NS
EPL1YFL024C1.410.55090.45083.02E−01NS
BUD27YFL023W0.4980.45017.99E−01NS
FMP45YDL222C1.380.52180.46845.82E−01NS
PET1YOR201C1.340.74550.78298.89E−01NS
HIS3YOR202W0.82940.74222.92E−01NS
ARG5,6YER069W1.290.24760.2708.14E−01NS
MOT3YMR070W1.260.95180.95756.64E−01NS
VHT1YGR065C1.190.6070.57043.81E−01NS
PHO89YBR296C1.120.1920.14992.40E−01NS
MGA1YGR249W1.110.30560.24722.93E−01NS
ECM22YLR228C1.080.91830.83741.90E−01NS
ARO1YDR127W1.050.53580.60155.77E−01NS
HIS7YBR248C0.980.41990.60994.36E−01NS
GUA1YMR217W0.960.8080.83983.36E−01NS
SKY1YMR216C0.67780.85011.62E−01NS
  • a As discussed in the text, we identified 56 Bas1p binding sites in sporulating cells. Fifteen of these sites were located between divergentally transcribed genes, resulting in 71 potential targets. We also measured the recombination activity of each ORF in the genome in both wild-type and bas1 mutant strains.

  • b Highest value (log2 ratio of hybridization to Bas1p-enriched DNA/total genomic DNA) for a 1-kb “window” within the binding site peak.

  • c Recombination activities were calculated by determining the ratio of hybridization to two hybridization probes (Spo11p-enriched DNA sample/total genomic DNA) and ranking these ratios for all ORFs and intergenic regions; the values shown are the median ranking, with 1 representing the “hottest” and 0 representing the “coldest.”

  • d Comparison of the rankings of samples in the wild-type (11 microarrays) and bas1 (10 samples) strains by a two-tailed t test, assigning a probability to the difference between the samples.

  • e Based on the p value, we determined whether the recombination activity of the ORF was significantly reduced (down), significantly elevated (up), or not significantly affected (NS).