TABLE 4.

Expression of GCN4-lacZ in Pol III transcription mutants

Strainβ-Galactosidase activity (U)a
NonstarvationStarvation
Expt 1
    WT (YPH500)45400
    rpc160-112250530
    tfc3-G349E190450
    tfc7N1180445
    brf1-II.6270490
Expt 2
    WT (YPH500)40425
    Δgcn21525
    tfc3-G349E180450
    tfc3-G349EΔgcn2150140
  • a The indicated strains, transformed with plasmid p180 (GCN4-lacZ-, CEN, URA), were grown at the permissive temperature under the nonstarvation or starvation conditions as described in Materials and Methods. Extracts were prepared and assayed for β-galactosidase activity (expressed as nanomoles of o-nitrophenol-β-d-galactopyranoside hydrolyzed per minute per microgram of total protein). The reported values are averages of at least three independent measurements, performed in duplicate, that differed by no more than 20% of the mean. The upper and lower parts of the table refer to two independent experiments in which different groups of strains were analyzed. WT, wild type.