TABLE 1.

RNA Pol III transcription mutant and background strains used in this study

StrainGenotypeSource or referencea
YNN282MATα ade2-1 his3-Δ200 lys2-801 trp1-Δ ura3-52 gal mal CUPrYGSC
YPH500MATα ade2-101 his3-Δ200 leu2-Δ1 lys2-801 trp1-Δ63 ura3-52YGSC
rpc160-112MATα ade2-101 his3-Δ200 leu2-Δ1 lys2-801 trp1-Δ63 ura3-52 rpc160Δ::HIS3 pC160-112 (TRP1 CEN4 rpc160-112)13
BRF1MATa leu2 ura3-52 brf1::HIS3 pRSM3 (LEU2 CEN BRF1)10
brf1-II.6MATa leu2 ura3-52 brf1::HIS3 pRSM3*II.6 (LEU2 CEN brf1-II.6)2
tfc3-G349EMATα ade1 his3-Δ200 leu2Δ1 lys2-801 trp1-Δ63 ura3-52 tsv11533
tfc7-ΔN1MATα ade2-101 his3-Δ200 lys2-801 trp1-Δ63 ura3-52 tfc7Δ::HIS3 pTFC7-ΔN1 (TRP1 CEN4 tfc7-ΔN1)37
  • a YGSC, Yeast Genetic Stock Center.